FasL active protein

FasL protein

Cat. Num. AAA536558

• Gene Names: FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
• Applications: User optimized
• Purity: > 98% by SDS-PAGE gel and HPLC Analyses.
• Synonyms: FasL; FasL protein; Apo I Ligand protein; CD95L protein; TNFSF6 protein; soluble Fas Ligand protein; APTL protein; sFASL protein; FasL active protein

• Host: Human
• Purity/Purification: > 98% by SDS-PAGE gel and HPLC Analyses.
• Form/Format: Sterile filtered through a 0.2 micron filter. Lyophilized from 1 x PBS, pH 7.2. Reconsitute in water to a concentration of 0.1-1.0 mg/ml
• Concentration: 10ug per vial (varies by lot)
• Sequence: Region of FasL protein corresponding to amino acids HHHHHHHHPS PPPEKKELRK VAHLTGKSNS RSMPLEWEDT YGIVLLSGVK YKKGGLVINE TGLYFVYSKV YFRGQSCNNL PLSHKVYMRN SKYPQDLVMM EGKMMSYCTT GQMWARSSYL GAVFNLTSAD HLYVNVSELS LVNFEESQTF FGLYKL.
• Sequence Length: 281

• Applicable Applications for FasL active protein: User optimized
• Application Notes: Specific activity is > 2 x 10^5 units/mg
• Protein Type: Recombinant
• Biological Significance: Fas and Fas Ligand (FasL) belong to the TNF superfamily and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD followed by processing of the pro-enzyme to active forms. These active caspases then cleave various cellular substrates leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL. The full length Fas (receptor) is a 319 amino acid type I transmembrane protein, which contains a 157 amino acid extracellular domain, a 17 amino acid transmembrane domain, and 145 amino acid cytoplasmic domain.
• Bioactivity: Determined by it's ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The ED50 for this effect is approximately 10.0 ng/ml, corresponding to a specific activity of approximately 1 x 10^5 units/mg.
• Endotoxin Levels: Endotoxin level is less than 0.1 ng per ug (1 EU/ug).
• Expression System: CHO cells
• Biohazard: Use standard laboratory precautions when handling this material.
• Preparation and Storage: Ships ambient or refrigerated. Upon receipt store at 4 degree C short term, -20 degree C long term. After reconsituted, the solution can be stored at 4 degree C for up to 1 week. Working aliquots stored with a carrier protein are stable for at least 12 months at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Related Product Information for FasL active protein

Purified soluble Human FasL protein

Product Categories/Family for FasL active protein

Cancer

NCBI and Uniprot Product Information

• NCBI GI #: 28569614
• NCBI GeneID: 356
• UniProt Accession #: P48023; Q9BZP9
• Molecular Weight: 17.6 kDa
• NCBI Official Full Name: Fas ligand
• NCBI Official Synonym Full Names: Fas ligand (TNF superfamily, member 6)
• NCBI Official Symbol: FASLG
• NCBI Official Synonym Symbols: APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 6
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 6
• UniProt Gene Name: FASLG
• UniProt Synonym Gene Names: APT1LG1; CD95L; FASL; TNFSF6; APTL; CD95-L; Fas ligand; FasL; sFasL; APL; FasL ICD; SPA
• UniProt Entry Name: TNFL6_HUMAN

NCBI Description

This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2014]

Uniprot Description

FasL: Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects. Homotrimer (Probable). Interacts with ARHGAP9, BAIAP2L1, BTK, CACNB3, CACNB4, CRK, DLG2, DNMBP, DOCK4, EPS8L3, FGR, FYB, FYN, HCK, ITK, ITSN2, KALRN, LYN, MACC1, MIA, MPP4, MYO15A, NCF1, NCK1, NCK2, NCKIPSD, OSTF1, PIK3R1, PSTPIP1, RIMBP3C, SAMSN1, SH3GL3, SH3PXD2B, SH3PXD2A, SH3RF2, SKAP2, SNX33, SNX9, SORBS3, SPTA1, SRC, SRGAP1, SRGAP2, SRGAP3, TEC, TJP3 and YES1. Belongs to the tumor necrosis factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1q23

Cellular Component: lysosomal lumen; extracellular space; integral to plasma membrane; perinuclear region of cytoplasm; plasma membrane; extracellular region; caveola; nucleus; external side of plasma membrane

Molecular Function: protein binding; death receptor binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: caspase activation; positive regulation of I-kappaB kinase/NF-kappaB cascade; transcription, DNA-dependent; apoptosis; positive regulation of apoptosis; response to lipopolysaccharide; negative regulation of transcription from RNA polymerase II promoter; signal transduction; cellular chloride ion homeostasis; endosomal lumen acidification; inflammatory cell apoptosis; negative regulation of angiogenesis; induction of apoptosis via death domain receptors; cell-cell signaling; positive regulation of neuron apoptosis; positive regulation of epidermal growth factor receptor signaling pathway; positive regulation of cell proliferation; retinal cell programmed cell death; immune response

Disease: Lung Cancer; Autoimmune Lymphoproliferative Syndrome