EG-VEGF Active Protein | PROK1 active protein

EG-VEGF, Recombinant, Human (Prokineticin-1, Endocrine-gland-derived Vascular Endothelial Growth Factor, Mambakine, PROK1, UNQ600/PRO1186)

Cat. Num. AAA637389

• Gene Names: PROK1; PK1; PRK1; EGVEGF
• Reactivity: Human
• Purity: Highly Purified; ~97% (RP-HPLC)
• Synonyms: EG-VEGF; Recombinant; Human (Prokineticin-1; Endocrine-gland-derived Vascular Endothelial Growth Factor; Mambakine; PROK1; UNQ600/PRO1186); PROK1 active protein

• Reactivity: Human
• Purity/Purification: Highly Purified; ~97% (RP-HPLC)
• Form/Format: Supplied as a lyophilized powder. Reconstitute with sterile water to 100ug/ml.
• Sequence: AVITGACERD VQCGAGTCCA ISLWLRGLRM CTPLGREGEE CHPGSHKVPF; FRKRKHHTCP CLPNLLCSRF PDGRYRCSMD LKNINF

• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC) using a concentration range of 1-5ng/ml.
• Endotoxin Level: ~0.01ng/ug (LAL)
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile buffer or dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for PROK1 active protein

Recombinant Human Endocrine Gland-Derived Vascular Endothelial Growth Factor (EG-hVEGF) is selectively expressed in steroidogenic glands and promotes the growth of endocrine gland endothelium. The identification of tissue-selective angiogenic factors raises the possibility that other secreted molecules in this class exist. Consistent with such an expression pattern, the human EG-VEGF gene promoter has a potential binding site for steroidogenic factor-1, a pivotal element for steroidogenic-specific transcription. In the human ovary, the expression of EG-VEGF is temporally and spatially complementary to the expression of VEGF-A, both in the follicular and in the luteal phase, suggesting complementary and coordinated roles of these molecules in ovarian angiogenesis. Also, EG-VEGF expression correlates with vascularity in the polycystic ovary syndrome, a leading cause of infertility.

Product Categories/Family for PROK1 active protein

Growth Factors; Cytokines; Growth Factors-VEGF

NCBI and Uniprot Product Information

• NCBI GI #: 37182488
• NCBI GeneID: 84432
• UniProt Accession #: P58294; Q5VWD4; Q8TC69
• Molecular Weight: ~9.6kD
• NCBI Official Full Name: EG-VEGF
• NCBI Official Synonym Full Names: prokineticin 1
• NCBI Official Symbol: PROK1
• NCBI Official Synonym Symbols: PK1; PRK1; EGVEGF
• NCBI Protein Information: prokineticin-1; EG-VEGF; mambakine; black mamba toxin-related protein; endocrine-gland-derived vascular endothelial growth factor
• UniProt Protein Name: Prokineticin-1
• Protein Family: Prokineticin
• UniProt Gene Name: PROK1
• UniProt Synonym Gene Names: EG-VEGF
• UniProt Entry Name: PROK1_HUMAN

NCBI Description

The protein encoded by this gene induces proliferation, migration, and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. It has little or no effect on a variety of other endothelial and non-endothelial cell types. Its expression is restricted to the steroidogenic glands (ovary, testis, adrenal, and placenta), is induced by hypoxia, and often complementary to the expression of vascular endothelial growth factor (VEGF), suggesting that these molecules function in a coordinated manner. [provided by RefSeq, Sep 2011]

Uniprot Description

PROK1: Potently contracts gastrointestinal (GI) smooth muscle. Induces proliferation, migration and fenestration (the formation of membrane discontinuities) in capillary endothelial cells derived from endocrine glands. Has little or no effect on a variety of other endothelial and non-endothelial cell types. Induces proliferation and differentiation, but not migration, of enteric neural crest cells. Directly influences neuroblastoma progression by promoting the proliferation and migration of neuroblastoma cells. Positively regulates PTGS2 expression and prostaglandin synthesis. May play a role in placentation. May play a role in normal and pathological testis angiogenesis. Belongs to the AVIT (prokineticin) family.

Protein type: Secreted; Cell cycle regulation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: extracellular region

Molecular Function: growth factor activity

Biological Process: activation of MAPK activity; positive regulation of cell division; positive regulation of cell proliferation; angiogenesis; regulation of angiogenesis