Heat Shock 70kDa Binding Protein 1 (HSPBP1) Recombinant Protein | HSPBP1 recombinant protein

Recombinant Heat Shock 70kDa Binding Protein 1 (HSPBP1)

Cat. Num. AAA2018593

• Gene Names: HSPBP1; FES1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Heat Shock 70kDa Binding Protein 1 (HSPBP1); Recombinant Heat Shock 70kDa Binding Protein 1 (HSPBP1); HSPBP1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.; MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-NMDNA ADFCQLSGMH LLVGRYLEAG AAGLRWRAAQ LIGTCSQNVA AIQEQVLGLG ALRKLLRLLD RDACDTVRVK ALFAISCLVR EQEAGLLQFL RLDGFSVLMR AMQQQVQKLK VKSAFLLQNL LVGHPEHKGT LCSMGMVQQL VALVRTEHSP FHEHVLGALC SLVTDFPQGV RECREPELGLEELLRHRCQL LQQHEEYQEE LEFCEKLLQT CFSS
• Sequence Length: 359

• Applicable Applications for HSPBP1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Asn136~Ser354 (Accession # Q9NZL4) with two N-terminal Tags, His-tag and GST-tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.3
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 194294548
• NCBI GeneID: 23640
• NCBI Accession #: NP_001123578.1
• NCBI GenBank Nucleotide #: NM_001130106.1
• UniProt Accession #: Q9NZL4; O95351; Q6ZNU5; B3KQP0
• Molecular Weight: 57.0kDa
• NCBI Official Full Name: hsp70-binding protein 1
• NCBI Official Synonym Full Names: HSPA (heat shock 70kDa) binding protein, cytoplasmic cochaperone 1
• NCBI Official Symbol: HSPBP1
• NCBI Official Synonym Symbols: FES1
• NCBI Protein Information: hsp70-binding protein 1; hspBP2; Hsp70 binding protein 1; hsp70-binding protein 2; hsp70 interacting protein; hsp70-interacting protein 1; hsp70-interacting protein 2; heat shock protein-binding protein 1
• UniProt Protein Name: Hsp70-binding protein 1
• Protein Family: Hsp70-binding protein
• UniProt Gene Name: HSPBP1
• UniProt Synonym Gene Names: HSPBP; HspBP1; HspBP2
• UniProt Entry Name: HPBP1_HUMAN

Uniprot Description

HSPBP1: Inhibits HSPA1A chaperone activity by changing the conformation of the ATP-binding domain of HSPA1A and interfering with ATP binding. Interferes with ubiquitination mediated by STUB1 and inhibits chaperone-assisted degradation of immature CFTR. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 19q13.42

Molecular Function: enzyme inhibitor activity; protein binding

Biological Process: protein folding; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; negative regulation of catalytic activity