SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Reg4 recombinant protein
Recombinant Reg4
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Reg4; Recombinant Reg4; Regenerating islet-derived protein 4 isoform 1; REG4; GISP; REG-IV; RELP; Reg4 recombinant protein
Host
Baculovirus
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid; In Phosphate Buffered Saline (pH 7.4) Containing 10% Glycerol.
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
23-158aa
Sequence
ADPDIIMRPS CAPGWFYHKS NCYGYFRKLR NWSDAELECQ SYGNGAHLAS ILSLKEASTI AEYISGYQRS QPIWIGLHDP QKRQQWQWID GAMYLYRSWS GKSMGGNKHC AEMSSNNNFL TWSSNECNKR QHFLCKYRPH HHHHH
Sequence Length
134
Applicable Applications for Reg4 recombinant protein
SDS-PAGE
Species
Human
Endotoxin
<1.0 EU per 1ug of protein (determined by LAL method)
Tag
His-Tag
Predicted N-Terminal
Ala
Preparation and Storage
Can be stored at 2 degree C to 8 degree C for 1 week. For long term storage, aliquot and store at -20 degree C to -80 degree C. Avoid repeated freezing and thawing cycles.
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
SDS-Page
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain )
Related Product Information for Reg4 recombinant protein
Reg4, also known regenerating islet-derived protein 4, a member of the regenerating gene family belonging to the calcium (C-type) dependent lectin superfamily. It is calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. This protein is involved in inflammatory and metaplastic responses because expression of this protein is increased within or near inflammation, dysplasia and metaplasia of the GI epithelium. And this protein is highly expressed in the gastrointestinal tract and markedly up-regulated in colon adenocarcinoma, pancreatic cancer, gastric adenocarcinoma, and inflammatory bowel disease. Recombinant human Reg4 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Categories/Family for Reg4 recombinant protein
References
Kawasaki Y., et al, (2015) Sci Rep. 5:14291. Jin J., et al, (2017) Med Sci Monit. 23:3715-3721.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
17.0kDa (145aa); 13.5-18KDa (SDS-PAGE under reducing conditions)
NCBI Official Full Name
regenerating islet-derived protein 4 isoform 2
UniProt Protein Name
Regenerating islet-derived protein 4
Protein Family
UniProt Gene Name
REG4
UniProt Synonym Gene Names
GISP; RELP; REG-4; Reg IV
UniProt Entry Name
REG4_HUMAN
Uniprot Description
REG4: Calcium-independent lectin displaying mannose-binding specificity and able to maintain carbohydrate recognition activity in an acidic environment. May be involved in inflammatory and metaplastic responses of the gastrointestinal epithelium. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1p13.1-p12
Cellular Component: cytoplasm; extracellular region
Molecular Function: heparin binding; calcium ion binding
Similar Products
Product Notes
The Reg4 reg4 (Catalog #AAA201119) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 23-158aa. AAA Biotech's Reg4 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the Reg4 reg4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: ADPDIIMRPS CAPGWFYHKS NCYGYFRKLR NWSDAELECQ SYGNGAHLAS ILSLKEASTI AEYISGYQRS QPIWIGLHDP QKRQQWQWID GAMYLYRSWS GKSMGGNKHC AEMSSNNNFL TWSSNECNKR QHFLCKYRPH HHHHH. It is sometimes possible for the material contained within the vial of "Reg4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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