Mouse Heat Shock 70Kda Protein 4 ELISA Kit | HSPA4 elisa kit
Mouse Heat Shock 70Kda Protein 4 ELISA Kit
Gene Names
HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HS24/P52
Reactivity
Mouse
Synonyms
Heat Shock 70Kda Protein 4; Mouse Heat Shock 70Kda Protein 4 ELISA Kit; HSPA4 elisa kit
Reactivity
Mouse
Specificity
No significant cross-reactivity or interference between Mouse HSPA4 and analogues was observed.
Samples
Serum, Plasma, Tissue Homogenate, Feces and Urine
Assay Type
Sandwich
Detection Range
0.625 ng/ml - 20 ng/ml.
Sensitivity
0.1 ng/ml.
Intended Uses
This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated HSPA4 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse HSPA4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HSPA4 and HSPA4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HSPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Intra-assay Precision
Intra-assay CV (%) is less than 15%.
Inter-assay Precision
Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
Preparation and Storage
Store all reagents at 2-8 degree C
Related Product Information for HSPA4 elisa kit
Background: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated HSPA4 concentrations in Mouse serum, plasma and other body fluids. Using Purified Mouse HSPA4 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add HSPA4 and HSPA4 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of HSPA4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
94,331 Da
NCBI Official Full Name
heat shock 70 kDa protein 4
NCBI Official Synonym Full Names
heat shock 70kDa protein 4
NCBI Official Symbol
HSPA4
NCBI Official Synonym Symbols
RY; APG-2; HSPH2; hsp70; hsp70RY; HS24/P52
NCBI Protein Information
heat shock 70 kDa protein 4; hsp70 RY; heat shock 70kD protein 4; heat shock protein, 110 kDa; heat shock 70-related protein APG-2
UniProt Protein Name
Heat shock 70 kDa protein 4
Protein Family
UniProt Gene Name
HSPA4
UniProt Synonym Gene Names
APG2
UniProt Entry Name
HSP74_HUMAN
Uniprot Description
HSP70RY: Interacts with TJP1/ZO-1. Belongs to the heat shock protein 70 family.
Protein type: Chaperone; Heat shock protein
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: mitochondrion; cytosol
Molecular Function: ATP binding
Biological Process: chaperone-mediated protein complex assembly; protein import into mitochondrial outer membrane; response to unfolded protein
Research Articles on HSPA4
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Product Notes
The Mouse HSPA4 hspa4 (Catalog #AAA035286) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA035286 ELISA Kit recognizes Mouse HSPA4. It is sometimes possible for the material contained within the vial of "Heat Shock 70Kda Protein 4, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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