Mouse anti-Human SNAPIN Monoclonal Antibody | anti-SNAPIN antibody

Anti-SNAPIN Antibody (clone 3H1)

Cat. Num. AAA2400651

• Gene Names: SNAPIN; BLOS7; BORCS3; SNAPAP; BLOC1S7
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry, Western Blot
• Purity: Purified from ascites by Protein A
• Synonyms: SNAPIN; Monoclonal Antibody; Anti-SNAPIN Antibody (clone 3H1); Mouse Monoclonal [clone 3H1] (IgG1; k) to Human SNAPIN; BLOC1S7; SNAP-associated protein; SNAP-25-binding protein; SNAP25BP; SNAPAP; anti-SNAPIN antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1, k
• Clone Number: 3H1
• Specificity: Human SNAPIN
• Purity/Purification: Purified from ascites by Protein A
• Form/Format: PBS, pH 7.4
• Sequence Length: 136

• Applicable Applications for anti-SNAPIN antibody: ELISA (EIA), Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 3-5 ug/ml; WB: 1:500-1:1000
• Immunogen: SNAPAP (NP_036569, 41 a.a. ~ 137 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C or lower.; Aliquot to avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-SNAPIN antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

Immunohistochemistry (WB)

(Anti-SNAPIN antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3 ug/ml.)

ELISA (EIA)

(Detection limit for recombinant GST tagged SNAPIN is 1 ng/ml as a capture antibody.)

Related Product Information for anti-SNAPIN antibody

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet densegranules and melanosomes. In concert with the AP-3 complex, the BLOC-1complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is alsoproposed to be involved in neurite extension.

NCBI and Uniprot Product Information

• NCBI GI #: 6912674
• NCBI GeneID: 23557
• NCBI Accession #: NP_036569.1
• NCBI GenBank Nucleotide #: NM_012437.5
• UniProt Accession #: O95295; Q5SXU8; D3DV56
• Molecular Weight: 14,874 Da
• NCBI Official Full Name: SNARE-associated protein Snapin
• NCBI Official Synonym Full Names: SNAP associated protein
• NCBI Official Symbol: SNAPIN
• NCBI Official Synonym Symbols: BLOS7; BORCS3; SNAPAP; BLOC1S7
• NCBI Protein Information: SNARE-associated protein Snapin
• UniProt Protein Name: SNARE-associated protein Snapin
• Protein Family: SNAPIN protein
• UniProt Gene Name: SNAPIN
• UniProt Synonym Gene Names: BLOC1S7; SNAP25BP; SNAPAP; BLOC-1 subunit 7; SNAP-associated protein

NCBI Description

The protein encoded by this gene is a coiled-coil-forming protein that associates with the SNARE (soluble N-ethylmaleimide-sensitive fusion protein attachment protein receptor) complex of proteins and the BLOC-1 (biogenesis of lysosome-related organelles) complex. Biochemical studies have identified additional binding partners. As part of the SNARE complex, it is required for vesicle docking and fusion and regulates neurotransmitter release. The BLOC-1 complex is required for the biogenesis of specialized organelles such as melanosomes and platelet dense granules. Mutations in gene products that form the BLOC-1 complex have been identified in mouse strains that are models of Hermansky-Pudlak syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2012]

Uniprot Description

Component of the BLOC-1 complex, a complex that is required for normal biogenesis of lysosome-related organelles (LRO), such as platelet dense granules and melanosomes. In concert with the AP-3 complex, the BLOC-1 complex is required to target membrane protein cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals. The BLOC-1 complex, in association with SNARE proteins, is also proposed to be involved in neurite extension. Plays a role in intracellular vesicle trafficking and synaptic vesicle recycling. May modulate a step between vesicle priming, fusion and calcium-dependent neurotransmitter release through its ability to potentiate the interaction of synaptotagmin with the SNAREs and the plasma-membrane-associated protein SNAP25. Its phosphorylation state influences exocytotic protein interactions and may regulate synaptic vesicle exocytosis. May also have a role in the mechanisms of SNARE-mediated membrane fusion in non-neuronal cells (PubMed:17182842, PubMed:18167355). As part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. Associated with the cytosolic face of lysosomes, the BORC complex may recruit ARL8B and couple lysosomes to microtubule plus-end-directed kinesin motor (PubMed:25898167).