HSPA2 / Heat Shock-Related 70 kDa Protein 2 Recombinant Protein | HSPA2 recombinant protein

Human HSPA2 / Heat Shock-Related 70 kDa Protein 2 Recombinant Protein

• Gene Names: HSPA2; HSP70-2; HSP70-3
• Applications: ELISA, Western Blot
• Purity: >95% by SDS-PAGE
• Synonyms: HSPA2 / Heat Shock-Related 70 kDa Protein 2; Human HSPA2 / Heat Shock-Related 70 kDa Protein 2 Recombinant Protein; Heat shock-related 70 kDa protein 2; Heat shock 70 kDa protein 2; HSPA2 recombinant protein

• Host: E Coli
• Purity/Purification: >95% by SDS-PAGE
• Form/Format: Liquid; 50mM NaH2PO4, 500mM NaCl Buffer with 500mM Imidazole, 10% Glycerol (PH8.0)
• Concentration: Reconstitution Dependent (varies by lot)
• Sequence Length: 639

• Applicable Applications for HSPA2 recombinant protein: ELISA (EIA), Western Blot (WB), Antigen Protein (AP), Protein Array
• Species: Human
• Tag: His
• Subunit: Interacts with FKBP6 (PubMed:18529014). Interacts with ZNF541. Component of the CatSper complex. Interacts with RABL2/RABL2A; binds preferentially to GTP-bound RABL2. Interacts with SHCBP1L; this interaction may promote the recruitment of HSPA2 to the spindle (By similarity). Interacts with FKBP6 (PubMed:18529014).
• Entry Function: Molecular chaperone implicated in a wide variety of cellular processes, including protection of the proteome from stress, folding and transport of newly synthesized polypeptides, activation of proteolysis of misfolded proteins and the formation and dissociation of protein complexes. Plays a pivotal role in the protein quality control system, ensuring the correct folding of proteins, the re-folding of misfolded proteins and controlling the targeting of proteins for subsequent degradation. This is achieved through cycles of ATP binding, ATP hydrolysis and ADP release, mediated by co-chaperones. The affinity for polypeptides is regulated by its nucleotide bound state. In the ATP-bound form, it has a low affinity for substrate proteins. However, upon hydrolysis of the ATP to ADP, it undergoes a conformational change that increases its affinity for substrate proteins. It goes through repeated cycles of ATP hydrolysis and nucleotide exchange, which permits cycles of substrate binding and release (PubMed:26865365). Plays a role in spermatogenesis. In association with SHCBP1L may participate in the maintenance of spindle integrity during meiosis in male germ cells.
• Preparation and Storage: Store at -20 degree C. Avoid repeated freezing and thawing.

NCBI and Uniprot Product Information

• NCBI GI #: 1708307
• NCBI GeneID: 3306
• UniProt Accession #: P54652
• NCBI Official Full Name: Heat shock-related 70 kDa protein 2
• NCBI Official Synonym Full Names: heat shock protein family A (Hsp70) member 2
• NCBI Official Symbol: HSPA2
• NCBI Official Synonym Symbols: HSP70-2; HSP70-3
• NCBI Protein Information: heat shock-related 70 kDa protein 2
• UniProt Protein Name: Heat shock-related 70 kDa protein 2
• Protein Family: Heat shock-related 70 kDa protein
• UniProt Gene Name: HSPA2
• UniProt Synonym Gene Names: Heat shock 70 kDa protein 2
• UniProt Entry Name: HSP72_HUMAN

Uniprot Description

HSPA2: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Interacts with ZNF541. Component of the CatSper complex. Belongs to the heat shock protein 70 family.

Protein type: Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: male germ cell nucleus; cell surface; mitochondrion; membrane; synaptonemal complex; nucleus; cytosol

Molecular Function: protein binding; enzyme binding; unfolded protein binding; glycolipid binding; ATP binding

Biological Process: G2/M-specific positive regulation of cyclin-dependent protein kinase activity; male meiosis I; protein refolding; male meiosis; response to unfolded protein; spermatid development