Western Blot (WB)
(Western Blot analysis of OTUB2 expression in transfected 293T cell line by OTUB2 polyclonal antibody. Lane 1: OTUB2 transfected lysate (27.2kD). Lane 2: Non-transfected lysate.)
Rabbit anti-Human OTUB2 Polyclonal Antibody | anti-OTUB2 antibody
OTUB2 (Ubiquitin Thioesterase OTUB2, Deubiquitinating Enzyme OTUB2, OTU Domain-containing Ubiquitin Aldehyde-binding Protein 2, Otubain-2, Ubiquitin-specific-processing Protease OTUB2, C14orf137, OTB2, OTU2, FLJ21916, MGC3102) (FITC)
Gene Names
OTUB2; OTB2; OTU2; C14orf137
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
OTUB2; Polyclonal Antibody; OTUB2 (Ubiquitin Thioesterase OTUB2; Deubiquitinating Enzyme OTUB2; OTU Domain-containing Ubiquitin Aldehyde-binding Protein 2; Otubain-2; Ubiquitin-specific-processing Protease OTUB2; C14orf137; OTB2; OTU2; FLJ21916; MGC3102) (FITC); anti-OTUB2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human OTUB2.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-OTUB2 antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
Full length human OTUB2, aa1-234 (NP_075601.1).
Immunogen Sequence
MSETSFNLISEKCDILSILRDHPENRIYRRKIEELSKRFTAIRKTKGDGNCFYRALGYSYLESLLGKSREIFKFKERVLQTPNDLLAAGFEEHKFRNFFNAFYSVVELVEKDGSVSSLLKVFNDQSASDHIVQFLRLLTSAFIRNRADFFRHFIDEEMDIKDFCTHEVEPMATECDHIQITALSQALSIALQVEYVDEMDTALNHHVFPEAATPSVYLLYKTSHYNILYAADKH
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis of OTUB2 expression in transfected 293T cell line by OTUB2 polyclonal antibody. Lane 1: OTUB2 transfected lysate (27.2kD). Lane 2: Non-transfected lysate.)
Western Blot (WB)
(Western Blot analysis of OTUB2 expression in transfected 293T cell line by OTUB2 polyclonal antibody. Lane 1: OTUB2 transfected lysate (27.2kD). Lane 2: Non-transfected lysate.)
Related Product Information for anti-OTUB2 antibody
Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'-and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains.
Product Categories/Family for anti-OTUB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
8,637 Da
NCBI Official Full Name
ubiquitin thioesterase OTUB2
NCBI Official Synonym Full Names
OTU deubiquitinase, ubiquitin aldehyde binding 2
NCBI Official Symbol
OTUB2
NCBI Official Synonym Symbols
OTB2; OTU2; C14orf137
NCBI Protein Information
ubiquitin thioesterase OTUB2; OTU domain, ubiquitin aldehyde binding 2; OTU domain-containing ubiquitin aldehyde-binding protein 2; deubiquitinating enzyme OTUB2; otubain-2; ubiquitin-specific protease otubain 2; ubiquitin-specific-processing protease OTU
UniProt Protein Name
Ubiquitin thioesterase OTUB2
Protein Family
UniProt Gene Name
OTUB2
UniProt Synonym Gene Names
C14orf137; OTB2; OTU2
UniProt Entry Name
OTUB2_HUMAN
NCBI Description
This gene encodes one of several deubiquitylating enzymes. Ubiquitin modification of proteins is needed for their stability and function; to reverse the process, deubiquityling enzymes remove ubiquitin. This protein contains an OTU domain and binds Ubal (ubiquitin aldehyde); an active cysteine protease site is present in the OTU domain. [provided by RefSeq, Aug 2011]
Uniprot Description
OTUB2: Hydrolase that can remove conjugated ubiquitin from proteins in vitro and may therefore play an important regulatory role at the level of protein turnover by preventing degradation. Mediates deubiquitination of both 'Lys-48'- and 'Lys-63'-linked polyubiquitin chains, with a preference for 'Lys-63'-linked polyubiquitin chains. Belongs to the peptidase C65 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: EC 3.4.19.12; Hydrolase; Ubiquitin-specific protease
Chromosomal Location of Human Ortholog: 14q32.12
Cellular Component: nucleus
Molecular Function: ubiquitin-specific protease activity
Biological Process: protein deubiquitination; proteolysis
Research Articles on OTUB2
Similar Products
Product Notes
The OTUB2 otub2 (Catalog #AAA6388143) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OTUB2 (Ubiquitin Thioesterase OTUB2, Deubiquitinating Enzyme OTUB2, OTU Domain-containing Ubiquitin Aldehyde-binding Protein 2, Otubain-2, Ubiquitin-specific-processing Protease OTUB2, C14orf137, OTB2, OTU2, FLJ21916, MGC3102) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's OTUB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the OTUB2 otub2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "OTUB2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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