Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat BLVRB Polyclonal Antibody | anti-BLVRB antibody
Goat anti-BLVRB (aa38-49) Antibody
Gene Names
BLVRB; FLR; BVRB; SDR43U1; HEL-S-10
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
BLVRB; Polyclonal Antibody; Goat anti-BLVRB (aa38-49) Antibody; Goat Anti-BLVRB Antibody; biliverdin reductase B (flavin reductase (NADPH)); BVRB; FLR; SDR43U1; BVR-B; FR; GHBP; NADPH-dependent diaphorase; NADPH-flavin reductase; biliverdin-IX beta-reductase; flavin reductase (NADPH); green heme-binding protein; short chain dehydrogenas; BLVRB antibody; biliverdin reductase B (flavin reductase (NADPH)) antibody; BVRB antibody; FLR antibody; SDR43U1 antibody; BVR-B antibody; FR antibody; GHBP antibody; NADPH-dependent diaphorase antibody; NADPH-flavin reductase antibody; biliverdin-IX beta-reductase antibody; flavin reductase (NADPH) antibody; green heme-binding protein antibody; short chain dehydrogenas antibody; BLVRB (aa38-49); anti-BLVRB antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
SRLPSEGPRPAH
Sequence Length
206
Applicable Applications for anti-BLVRB antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 128000.
Western Blot: Approx 23kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000704.1). Recommended concentration: 0.1-0.3ug/ml.
Western Blot: Approx 23kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000704.1). Recommended concentration: 0.1-0.3ug/ml.
Immunogen
Peptide with sequence C-SRLPSEGPRPAH, from the internal region of the protein sequence according to NP_000704.1.
Epitope
Internal region
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.1ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,119 Da
NCBI Official Full Name
flavin reductase (NADPH)
NCBI Official Synonym Full Names
biliverdin reductase B
NCBI Official Symbol
BLVRB
NCBI Official Synonym Symbols
FLR; BVRB; SDR43U1; HEL-S-10
NCBI Protein Information
flavin reductase (NADPH)
UniProt Protein Name
Flavin reductase (NADPH)
UniProt Gene Name
BLVRB
UniProt Synonym Gene Names
FLR; BVR-B; GHBP; FLR
UniProt Entry Name
BLVRB_HUMAN
NCBI Description
The final step in heme metabolism in mammals is catalyzed by the cytosolic biliverdin reductase enzymes A and B (EC 1.3.1.24).[supplied by OMIM, Jul 2009]
Uniprot Description
BLVRB: Broad specificity oxidoreductase that catalyzes the NADPH-dependent reduction of a variety of flavins, such as riboflavin, FAD or FMN, biliverdins, methemoglobin and PQQ (pyrroloquinoline quinone). Contributes to heme catabolism and metabolizes linear tetrapyrroles. Can also reduce the complexed Fe(3+) iron to Fe(2+) in the presence of FMN and NADPH. In the liver, converts biliverdin to bilirubin.
Protein type: Oxidoreductase; Cofactor and Vitamin Metabolism - porphyrin and chlorophyll; EC 1.3.1.24; EC 1.5.1.30
Chromosomal Location of Human Ortholog: 19q13.1-q13.2
Cellular Component: cytoplasm; cytosol; nucleoplasm; plasma membrane
Molecular Function: biliverdin reductase activity; flavin reductase activity
Biological Process: heme catabolic process
Research Articles on BLVRB
Similar Products
Product Notes
The BLVRB blvrb (Catalog #AAA423292) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-BLVRB (aa38-49) Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's BLVRB can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 23kDa band observed in Human Liver lysates (calculated MW of 22.1kDa according to NP_000704.1). Recommended concentration: 0.1-0.3ug/ml. Researchers should empirically determine the suitability of the BLVRB blvrb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SRLPSEGPRP AH. It is sometimes possible for the material contained within the vial of "BLVRB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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