Canine Heat Shock Protein 90 ELISA Kit | HSP-90 elisa kit

Canine Heat Shock Protein 90 ELISA Kit

Cat. Num. AAA740094

• Reactivity: Canine
• Synonyms: Heat Shock Protein 90; Canine Heat Shock Protein 90 ELISA Kit; HSP-90 elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSP-90 elisa kit

Intended Uses: This HSP90 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine HSP90. This ELISA kit for research use only!

Principle of the Assay: HSP90 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HSP90 antibody and an HSP90-HRP conjugate. The assay sample and buffer are incubated together with HSP90-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HSP90 concentration since HSP90 from samples and HSP90-HRP conjugate compete for the anti-HSP90 antibody binding site. Since the number of sites is limited, as more sites are occupied by HSP90 from the sample, fewer sites are left to bind HSP90-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP90 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HSP-90 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 83303658
• NCBI GeneID: 3505604
• Molecular Weight: 80,640 Da
• NCBI Official Full Name: Heat shock protein 90
• NCBI Official Symbol: AFUA_5G04170
• NCBI Protein Information: molecular chaperone and allergen Mod-E/Hsp90/Hsp1
• UniProt Protein Name: Heat shock protein 90
• Protein Family: Heat shock protein
• UniProt Gene Name: hsp90
• UniProt Synonym Gene Names: hsp1; AFUA_5G04170
• UniProt Entry Name: HSP90_ASPFU

Uniprot Description

Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function ().