Human Heat Shock Protein 90 ELISA Kit | HSP 90 elisa kit
Human Heat Shock Protein 90 ELISA Kit
Reactivity
Human
Synonyms
Heat Shock Protein 90; Human Heat Shock Protein 90 ELISA Kit; HSP 90 elisa kit
Reactivity
Human
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Competitive or Sandwich
Detection Range
0.5-10ng/mL
Sensitivity
0.1ng/mL
Intended Uses
This HSP-90 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human HSP-90. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for HSP 90 elisa kit
Principle of the assay: HSP-90 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for HSP-90. Standards or samples are then added to the microtiter plate wells and HSP-90 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of HSP-90 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for HSP-90 are added to each well to "sandwich" the HSP-90 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain HSP-90 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The HSP-90 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for HSP 90 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
80,640 Da
NCBI Official Full Name
Heat shock protein 90
NCBI Official Symbol
AFUA_5G04170
NCBI Protein Information
molecular chaperone and allergen Mod-E/Hsp90/Hsp1
UniProt Protein Name
Heat shock protein 90
Protein Family
UniProt Gene Name
hsp90
UniProt Synonym Gene Names
hsp1; AFUA_5G04170
UniProt Entry Name
HSP90_ASPFU
Uniprot Description
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function ().
