Heat Shock Protein Beta 3 (HSPb3) Recombinant Protein | HSPb3 recombinant protein

Recombinant Heat Shock Protein Beta 3 (HSPb3)

Cat. Num. AAA2011834

• Gene Names: HSPB3; HMN2C; DHMN2C; HSPL27
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Heat Shock Protein Beta 3 (HSPb3); Recombinant Heat Shock Protein Beta 3 (HSPb3); HSPb3 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSG SEF-MAKIILRHLI EIPVRYQEEF EARGLEDCRL DHALYALPGP TIVDLRKTRA AQSPPVDSAA ETPPREGKSH FQILLDVVQF LPEDIIIQTF EGWLLIKAQH GTRMDEHGFI SRSFTRQYKL PDGVEIKDLS AVLCHDGILV VEVKDPVGTK
• Sequence Length: 150

• Applicable Applications for HSPb3 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Met1~Lys150 (Accession # Q12988) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 5453688
• NCBI GeneID: 8988
• NCBI Accession #: NP_006299.1
• NCBI GenBank Nucleotide #: NM_006308.2
• UniProt Accession #: Q12988
• Molecular Weight: 18.5kDa
• NCBI Official Full Name: heat shock protein beta-3
• NCBI Official Synonym Full Names: heat shock 27kDa protein 3
• NCBI Official Symbol: HSPB3
• NCBI Official Synonym Symbols: HMN2C; DHMN2C; HSPL27
• NCBI Protein Information: heat shock protein beta-3; HSP 17; protein 3; heat shock 17 kDa protein
• UniProt Protein Name: Heat shock protein beta-3
• Protein Family: Heat shock protein
• UniProt Gene Name: HSPB3
• UniProt Synonym Gene Names: HSP27; HSPL27; HspB3; HSP 17
• UniProt Entry Name: HSPB3_HUMAN

NCBI Description

This gene encodes a muscle-specific small heat shock protein. A mutation in this gene is the cause of autosomal dominant distal hereditary motor neuropathy type 2C.[provided by RefSeq, Sep 2010]

Uniprot Description

HSPB3: Inhibitor of actin polymerization. Defects in HSPB3 are the cause of distal hereditary motor neuronopathy type 2C (HMN2C). It is a neuromuscular disorder. Distal hereditary motor neuronopathies constitute a heterogeneous group of neuromuscular disorders caused by selective degeneration of motor neurons in the anterior horn of the spinal cord, without sensory deficit in the posterior horn. The overall clinical picture consists of a classical distal muscular atrophy syndrome in the legs without clinical sensory loss. The disease starts with weakness and wasting of distal muscles of the anterior tibial and peroneal compartments of the legs. Later on, weakness and atrophy may expand to the proximal muscles of the lower limbs and/or to the distal upper limbs. Belongs to the small heat shock protein (HSP20) family.

Protein type: Heat shock protein

Chromosomal Location of Human Ortholog: 5q11.2

Cellular Component: cytoplasm; nucleus

Biological Process: response to unfolded protein

Disease: Neuronopathy, Distal Hereditary Motor, Type Iic