Rabbit anti-Human CYP21A2 Polyclonal Antibody | anti-CYP21A2 antibody

CYP21A2, ID (CYP21A2, CYP21, CYP21B, Steroid 21-hydroxylase, 21-OHase, Cytochrome P-450c21, Cytochrome P450 21, Cytochrome P450 XXI, Cytochrome P450-C21, Cytochrome P450-C21B) (FITC)

Cat. Num. AAA6290444

• Gene Names: CYP21A2; CAH1; CPS1; CA21H; CYP21; CYP21B; P450c21B
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: CYP21A2; Polyclonal Antibody; ID (CYP21A2; CYP21; CYP21B; Steroid 21-hydroxylase; 21-OHase; Cytochrome P-450c21; Cytochrome P450 21; Cytochrome P450 XXI; Cytochrome P450-C21; Cytochrome P450-C21B) (FITC); Cytochrome P450-C21B; anti-CYP21A2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human CYP21A2
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-CYP21A2 antibody: Flow Cytometry (FC/FACS), ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; ELISA: 1:1,000; IF: 1:10-50; IHC: 1:50-100; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: CYP21A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 224~253 amino acids from the Center region of human CYP21A2.
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of CYP21A2 Antibody (Center) in MDA-MB435 cell line lysates (35ug/lane). CYP21A2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma reacted with HADHA Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(CYP21A2 Antibody (Center) flow cytometric analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of CYP21A2 Antibody (Center) with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-CYP21A2 antibody

CYP21A2 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in CYP21A2 gene cause congenital adrenal hyperplasia.

Product Categories/Family for anti-CYP21A2 antibody

Antibodies; Enzymes; Cytochrome

References

Guerra-Junior, G., Clin. Exp. Immunol. 155 (2), 182-188 (2009). Abid, F., Clin. Chem. Lab. Med. 46 (12), 1707-1713 (2008). Nelson, D.R., Pharmacogenetics 14 (1), 1-18 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 323510663
• NCBI GeneID: 1589
• NCBI Accession #: NP_000491.4
• NCBI GenBank Nucleotide #: NM_000500.7
• UniProt Accession #: P08686; P04033; Q01204; Q08AG8; Q16749; Q16806; Q5ST44; Q96NU8; A2BHY6
• Molecular Weight: 55,887 Da
• NCBI Official Full Name: steroid 21-hydroxylase isoform a
• NCBI Official Synonym Full Names: cytochrome P450, family 21, subfamily A, polypeptide 2
• NCBI Official Symbol: CYP21A2
• NCBI Official Synonym Symbols: CAH1; CPS1; CA21H; CYP21; CYP21B; P450c21B
• NCBI Protein Information: steroid 21-hydroxylase; 21-OHase; cytochrome P450 21; cytochrome P450 XXI; cytochrome P450-C21B; steroid 21-monooxygenase; cytochrome P450, subfamily XXIA (steroid 21-hydroxylase, congenital adrenal hyperplasia), polypeptide 2
• UniProt Protein Name: Steroid 21-hydroxylase
• UniProt Gene Name: CYP21A2
• UniProt Synonym Gene Names: CYP21; CYP21B
• UniProt Entry Name: CP21A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and hydroxylates steroids at the 21 position. Its activity is required for the synthesis of steroid hormones including cortisol and aldosterone. Mutations in this gene cause congenital adrenal hyperplasia. A related pseudogene is located near this gene; gene conversion events involving the functional gene and the pseudogene are thought to account for many cases of steroid 21-hydroxylase deficiency. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP21A2: Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids. Defects in CYP21A2 are the cause of adrenal hyperplasia type 3 (AH3). AH3 is a form of congenital adrenal hyperplasia, a common recessive disease due to defective synthesis of cortisol. Congenital adrenal hyperplasia is characterized by androgen excess leading to ambiguous genitalia in affected females, rapid somatic growth during childhood in both sexes with premature closure of the epiphyses and short adult stature. Four clinical types: 'salt wasting' (SW, the most severe type), 'simple virilizing' (SV, less severely affected patients), with normal aldosterone biosynthesis, 'non-classic form' or late onset (NC or LOAH), and 'cryptic' (asymptomatic). Belongs to the cytochrome P450 family.

Protein type: EC 1.14.99.10; Oxidoreductase; Lipid Metabolism - C21-steroid hormone

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: endoplasmic reticulum membrane

Molecular Function: steroid 21-monooxygenase activity; iron ion binding; heme binding; steroid hydroxylase activity; steroid binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; mineralocorticoid biosynthetic process; glucocorticoid biosynthetic process; sterol metabolic process

Disease: Adrenal Hyperplasia, Congenital, Due To 21-hydroxylase Deficiency