Haptoglobin (Hpt) Recombinant Protein | Hpt recombinant protein

Recombinant Haptoglobin (Hpt)

Cat. Num. AAA2010214

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Haptoglobin (Hpt); Recombinant Haptoglobin (Hpt); Hpt recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- HHNLTSGA TLINEQWLLT TAKNLRLGHK NDTKAKDIAP TLRLYVGKKQ EVEIEKVIFH PDNSTVDIGL IKLKQKVPVN ERVMPICLPS KDYVNVGLVG YVSGWGRNAN LNFTEHLKYV MLPVADQEKC VQYYEGSTVP EKKTPKSPVG VQPILNEHTF CAGLSKYQED TCYGDAGSAF AVHDKDD
• Sequence Length: 347

• Applicable Applications for Hpt recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Sus scrofa; Porcine (Pig)
• Expression System: Prokaryotic expression
• Residues: His123~Asp307 (Accession # Q8SPS7) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 47522826
• NCBI GeneID: 397061
• NCBI Accession #: NP_999165.1
• NCBI GenBank Nucleotide #: NM_214000.2
• UniProt Accession #: Q8SPS7
• Molecular Weight: 22.2kDa
• NCBI Official Full Name: haptoglobin
• NCBI Official Symbol: HP
• NCBI Protein Information: haptoglobin; zonulin; haptoglobin alpha 1S
• UniProt Protein Name: Haptoglobin
• Protein Family: Hypoxanthine phosphoribosyltransferase
• UniProt Gene Name: HP
• UniProt Entry Name: HPT_PIG

Uniprot Description

HP: Haptoglobin combines with free plasma hemoglobin, preventing loss of iron through the kidneys and protecting the kidneys from damage by hemoglobin, while making the hemoglobin accessible to degradative enzymes. Defects in HP are the cause of anhaptoglobinemia (AHP). AHP is a condition characterized by the absence of the serum glycoprotein haptoglobin. Serum levels of haptoglobin vary among normal persons: levels are low in the neonatal period and in the elderly, differ by population, and can be influenced by environmental factors, such as infection. Secondary hypohaptoglobinemia can occur as a consequence of hemolysis, during which haptoglobin binds to free hemoglobin. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular region

Molecular Function: antioxidant activity; hemoglobin binding; catalytic activity

Biological Process: immune system process; metabolic process; defense response to bacterium; acute-phase response