Haptoglobin (Hpt) Recombinant Protein | Hpt recombinant protein

Recombinant Haptoglobin (Hpt)

Cat. Num. AAA2009405

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Haptoglobin (Hpt); Recombinant Haptoglobin (Hpt); Hpt recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- VVLH PNYSEVDIGL IKLKDKVPVN ERVMPICLPS KDYTEVGRVG YVSGWGRNSN FTYTDHLKYV MLPVADQDKC IQHYENSTVP ENKIPKNPVG VQPILNEHTF CAGMSKYQED TCYGDAGSTF AIHDLQQDTW YAAGILSFDK SCTVAEYGVY VKTFNILDWI QK
• Sequence Length: 347

• Applicable Applications for Hpt recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Val177~Lys342 (Accession # P19007) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 126723706
• NCBI GeneID: 100008816
• NCBI Accession #: NP_001075579.1
• NCBI GenBank Nucleotide #: NM_001082110.1
• UniProt Accession #: P19007; Q8MKG3; Q9MYU3
• Molecular Weight: 20.3kDa
• NCBI Official Full Name: haptoglobin
• NCBI Official Symbol: HP
• NCBI Protein Information: haptoglobin; zonulin
• UniProt Protein Name: Haptoglobin
• Protein Family: Hypoxanthine phosphoribosyltransferase
• UniProt Gene Name: HP
• UniProt Entry Name: HPT_RABIT

Uniprot Description

Function: As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidely cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway

By similarity.Uncleaved haptoglogin, also known as zonulin, plays a role in intestinal permeability, allowing intercellular tight junction disassembly, and controlling the equilibrium between tolerance and immunity to non-self antigens

By similarity.

Subunit structure: Tetramer of two alpha and two beta chains; disufide-linked. The Hemoglobin/haptoglobin complex is composed of a haptoglobin dimer bound to two hemoglobin alpha-beta dimers. Interacts with CD163

By similarity.

Subcellular location: Secreted.

Tissue specificity: Expressed by the liver and secreted in plasma.

Domain: The beta chain mediates most of the interactions with both subunits of hemoglobin, while the alpha chain forms the homodimeric interface

By similarity.

Sequence similarities: Belongs to the peptidase S1 family.Contains 1 peptidase S1 domain.Contains 1 Sushi (CCP/SCR) domain.

Caution: Although homologous to serine proteases, it has lost all essential catalytic residues and has no enzymatic activity.