Dog Parathyroid Hormone (PTH) ELISA Kit | PTH elisa kit

Dog Parathyroid Hormone (PTH) ELISA Kit

Cat. Num. AAA2700364

• Gene Names: PTH; PTH1
• Reactivity: Dog
• Synonyms: Parathyroid Hormone (PTH); Dog Parathyroid Hormone (PTH) ELISA Kit; iPTH; Intact Parathyroid Hormone; Parathormone; Parathyrin; PTH elisa kit

• Reactivity: Dog
• Specificity: This assay has high sensitivity and excellent specificity for detection of Parathyroid Hormone (PTH).; No significant cross-reactivity or interference between Parathyroid Hormone (PTH) and analogues was observed.
• Sequence Length: 115

• Assay Type: Competitive Inhibition
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and Other Biological Fluids
• Detection Range: 9.88-800pg/mL
• Sensitivity: < 4.15pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Parathyroid Hormone (PTH) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTH elisa kit

This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Parathyroid Hormone (PTH) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Parathyroid Hormone (PTH) and unlabeled Parathyroid Hormone (PTH) (Standards or samples) with the pre-coated antibody specific to Parathyroid Hormone (PTH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Parathyroid Hormone (PTH) in the sample.

NCBI and Uniprot Product Information

• NCBI GI #: 4506267
• NCBI GeneID: 5741
• NCBI Accession #: NP_000306.1
• NCBI GenBank Nucleotide #: NM_000315.3
• UniProt Accession #: P01270; Q4VB48; Q9UD38
• Molecular Weight: 12,861 Da
• NCBI Official Full Name: parathyroid hormone isoform 1 preproprotein
• NCBI Official Synonym Full Names: parathyroid hormone
• NCBI Official Symbol: PTH
• NCBI Official Synonym Symbols: PTH1
• NCBI Protein Information: parathyroid hormone
• UniProt Protein Name: Parathyroid hormone
• Protein Family: Parathyroid hormone
• UniProt Gene Name: PTH
• UniProt Synonym Gene Names: PTH

NCBI Description

This gene encodes a member of the parathyroid family of proteins. The encoded preproprotein is proteolytically processed to generate a protein that binds to the parathyroid hormone/parathyroid hormone-related peptide receptor and regulates blood calcium and phosphate levels. Excess production of the encoded protein, known as hyperparathyroidism, can result in hypercalcemia and kidney stones. On the other hand, defective processing of the encoded protein may lead to hypoparathyroidism, which can result in hypocalcemia and numbness. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2015]

Uniprot Description

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.