Bovine Hepatocyte Nuclear Factor 4 Alpha ELISA Kit | HNF4alpha elisa kit

Bovine Hepatocyte Nuclear Factor 4 Alpha ELISA Kit

Cat. Num. AAA745413

• Gene Names: HNF4A; TCF; HNF4; MODY; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
• Reactivity: Bovine
• Synonyms: Hepatocyte Nuclear Factor 4 Alpha; Bovine Hepatocyte Nuclear Factor 4 Alpha ELISA Kit; HNF4alpha elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This HNF4alpha ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine HNF4alpha. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HNF4alpha elisa kit

Principle of the Assay: HNF4alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-HNF4alpha antibody and an HNF4alpha-HRP conjugate. The assay sample and buffer are incubated together with HNF4alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the HNF4alpha concentration since HNF4alpha from samples and HNF4alpha-HRP conjugate compete for the anti-HNF4alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by HNF4alpha From the sample fewer sites are left to bind HNF4alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The comHNF4alpha concentration in each sample is interpolated from this standard curve.

Product Categories/Family for HNF4alpha elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 31077209
• NCBI GeneID: 3172
• NCBI Accession #: NP_849181.1
• NCBI GenBank Nucleotide #: NM_178850.2
• Molecular Weight: 43,988 Da
• NCBI Official Full Name: hepatocyte nuclear factor 4-alpha isoform HNF4alpha3
• NCBI Official Synonym Full Names: hepatocyte nuclear factor 4, alpha
• NCBI Official Symbol: HNF4A
• NCBI Official Synonym Symbols: TCF; HNF4; MODY; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha
• NCBI Protein Information: hepatocyte nuclear factor 4-alpha; TCF-14; HNF4alpha10/11/12; transcription factor 14; transcription factor HNF-4; hepatic nuclear factor 4 alpha; nuclear receptor subfamily 2 group A member 1
• UniProt Protein Name: Hepatocyte nuclear factor 4-alpha
• UniProt Gene Name: HNF4A
• UniProt Synonym Gene Names: HNF4; NR2A1; TCF14; HNF-4-alpha; TCF-14
• UniProt Entry Name: HNF4A_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear transcription factor which binds DNA as a homodimer. The encoded protein controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic genes. This gene may play a role in development of the liver, kidney, and intestines. Mutations in this gene have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Alternative splicing of this gene results in multiple transcript variants encoding several different isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

HNF4 alpha: a nuclear transcription factor which binds DNA as a homodimer. Controls the expression of several genes, including hepatocyte nuclear factor 1 alpha, a transcription factor which regulates the expression of several hepatic gene, alpha 1-antitrypsin, apolipoprotein CIII, and transthyretin genes. May be essential for development of the liver, kidney and intestine. Mutations have been associated with monogenic autosomal dominant non-insulin-dependent diabetes mellitus type I. Four alternatively spliced isoforms have been described.

Protein type: Nuclear receptor; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 20q13.12

Cellular Component: nucleoplasm; cytoplasm; nucleus

Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; ligand-dependent nuclear receptor activity; protein binding; protein homodimerization activity; DNA binding; zinc ion binding; steroid hormone receptor activity; fatty acid binding; transcription factor activity; receptor binding

Biological Process: transcription initiation from RNA polymerase II promoter; intracellular receptor-mediated signaling pathway; regulation of lipid metabolic process; positive regulation of transcription, DNA-dependent; lipid homeostasis; glucose homeostasis; endocrine pancreas development; regulation of transcription from RNA polymerase II promoter; negative regulation of cell proliferation; regulation of gastrulation; xenobiotic metabolic process; response to glucose stimulus; ornithine metabolic process; positive regulation of transcription from RNA polymerase II promoter; steroid hormone mediated signaling; gene expression; lipid metabolic process; negative regulation of cell growth; blood coagulation; regulation of insulin secretion; sex differentiation; phospholipid homeostasis

Disease: Fanconi Renotubular Syndrome 4 With Maturity-onset Diabetes Of The Young; Maturity-onset Diabetes Of The Young, Type 1; Diabetes Mellitus, Noninsulin-dependent