HO1 recombinant protein

HO1 recombinant protein

Cat. Num. AAA515269

• Gene Names: HMOX1; HO-1; HSP32; HMOX1D; bK286B10
• Applications: Western Blot
• Synonyms: HO1; HO1 recombinant protein; Heme Oxygenase (Decycling) 1; HMOX1; HSP32; bK286B10

• Host: E Coli
• Form/Format: 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
• Sequence Length: 1606

• Applicable Applications for HO1 recombinant protein: Western Blot (WB)
• Type: Recombinant Fusion Protein
• Species: Human
• Tag Information: GST tag
• Expression System: E.coli
• Source Note: Recombinant full-length human HO1 was expressed in E. coli cells
• Preparation and Storage: Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

SDS-PAGE

Related Product Information for HO1 recombinant protein

Recombinant full-length human HO1 was expressed in E. coli cells using an N-terminal GST tag

Scientific Background: HO1 (Heme oxygenase 1) catalyzes the oxidative cleavage of heme to biliverdin and is one of the main genes controlling heme synthesis and catabolism. HO1 plays a protective role in various disorders and can ameliorate experimental MN via multiple pathways, including anti-oxidative and immunomodulatory effects (1). Exposure of primary hepatocytes to carbon monoxide and nitric oxide results in dramatic induction of HO1 in dose- and time-dependent manner and this induction is blocked by MAP kinase inhibitors (MAPKs) but not by inhibitors of PI3 kinase pathway (2).

Product Categories/Family for HO1 recombinant protein

Cell Stress & Chaperone Proteins; Signaling Proteins - Cell Stress & Chaperone Proteins

References

1.Chia-Chao, W.u. et al: HO-1 induction ameliorates experimental murine membranous nephropathy: anti-oxidative, anti-apoptotic and immunomodulatory effects. Nephrology Dialysis Transplantation, 2008; 23(10):3082-3090.
2.Lee, B S. et al:. Carbon monoxide mediates heme oxygenase 1 induction via Nrf2 activation in hepatoma cells. Biochem Biophys Res Commun. 2006 May 12;343(3):965-72.

NCBI and Uniprot Product Information

• NCBI GI #: 298676487
• NCBI GeneID: 3162
• NCBI Accession #: NP_002124.1
• NCBI GenBank Nucleotide #: NM_002133
• UniProt Accession #: P09601
• Molecular Weight: ~56 kDa
• NCBI Official Full Name: Homo sapiens heme oxygenase (decycling) 1 (HMOX1), mRNA
• NCBI Official Synonym Full Names: heme oxygenase (decycling) 1
• NCBI Official Symbol: HMOX1
• NCBI Official Synonym Symbols: HO-1; HSP32; HMOX1D; bK286B10
• NCBI Protein Information: heme oxygenase 1; heat shock protein, 32-kD
• UniProt Protein Name: Heme oxygenase 1
• UniProt Gene Name: HMOX1
• UniProt Synonym Gene Names: HO; HO1; HO-1
• UniProt Entry Name: HMOX1_HUMAN

NCBI Description

Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.

Catalytic activity: Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.

Subcellular location: Microsome. Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side Ref.13.

Tissue specificity: Expressed at higher levels in renal cancer tissue than in normal tissue (at protein level). Ref.10

Induction: Heme oxygenase 1 activity is highly inducible by its substrate heme and by various non-heme substances such as heavy metals, bromobenzene, endotoxin, oxidizing agents and UVA.

Involvement in disease: Heme oxygenase 1 deficiency (HMOX1D) [MIM:614034]: A disease characterized by impaired stress hematopoiesis, resulting in marked erythrocyte fragmentation and intravascular hemolysis, coagulation abnormalities, endothelial damage, and iron deposition in renal and hepatic tissues. Clinical features include persistent hemolytic anemia, asplenia, nephritis, generalized erythematous rash, growth retardation and hepatomegaly.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7

Sequence similarities: Belongs to the heme oxygenase family.