HIPK3 recombinant protein

Recombinant HIPK3 (163-562) protein

Cat. Num. AAA389408

• Gene Names: HIPK3; PKY; YAK1; DYRK6; FIST3
• Synonyms: HIPK3; Recombinant HIPK3 (163-562) protein; Homeodomain Interacting Protein Kinase 3; DYRK6; FIST3; YAK1; HIPK3 recombinant protein

• Host: Baculovirus
• Form/Format: Recombinant HIPK3 (163-562) protein is supplied in 25mM HEPESNaOH pH7.5, 300mM NaCl, 10% glycerol, 0.04% Triton X-100, and 0.5mM TCEP.

• Protein Species: Human
• Tag: DYKDDDDK-Tag
• Protein Details: Recombinant HIPK3 (163-562) protein that includes amino acids 163-562 of human HIPK3 protein (accession number NP_005725.3) was expressed in a baculovirus expression system, and contains an N-terminal DYKDDDDK tag.
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant HIPK3 (163-562) protein 10% SDS-PAGE gel with Coomassie blue staining MW: 46.6 kDa Purity: >95%)

Testing Data

(HTRF assay for HIPK3 (163-562) activity 1 uM STK S3 substrate was incubated with different concentrations of HIPK3 (163-562) protein in a 10 ul reaction system containing 1×Enzymatic Buffer, 5 mM MgCl2, 1 mM DTT, and 100 ?M ATP for 1 hour. Then 10 ul detection reagents containing STK antibody (1:2) and SA-XL665 (1:100) diluted with 1× Detection Buffer were added and incubated with the reactions for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)

Related Product Information for HIPK3 recombinant protein

Background: HIPK3 (Homeodomain Interacting Protein Kinase 3), also known as FIST. It is a serine/threonine-protein kinase involved in transcription regulation, apoptosis and steroidogenic gene expression, HIPK3 phosphorylates JUN and RUNX2. It seems to negatively regulate apoptosis by promoting FADD phosphorylation and enhances androgen receptormediated transcription. It may also act as a transcriptional co-repressor for NK homeodomain transcription factors. In osteoblasts, HIPK3 supports transcription activation and phosphorylates RUNX2 that synergizes with SPEN/MINT to enhance FGFR2 mediated activation of the osteocalcin FGF-responsive element (OCFRE).

Product Categories/Family for HIPK3 recombinant protein

Recombinant Proteins; Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 114796624
• NCBI GeneID: 10114
• NCBI Accession #: NP_005725.3
• NCBI GenBank Nucleotide #: NM_005734.4
• UniProt Accession #: Q9H422; O14632; Q2PBG4; Q2PBG5; Q92632; Q9HAS2
• Molecular Weight: 131,256 Da
• NCBI Official Full Name: homeodomain-interacting protein kinase 3 isoform 1
• NCBI Official Synonym Full Names: homeodomain interacting protein kinase 3
• NCBI Official Symbol: HIPK3
• NCBI Official Synonym Symbols: PKY; YAK1; DYRK6; FIST3
• NCBI Protein Information: homeodomain-interacting protein kinase 3; ANPK; FIST; homolog of protein kinase YAK1; fas-interacting serine/threonine-protein kinase; androgen receptor-interacting nuclear protein kinase
• UniProt Protein Name: Homeodomain-interacting protein kinase 3
• Protein Family: Homeodomain-interacting protein kinase
• UniProt Gene Name: HIPK3
• UniProt Synonym Gene Names: DYRK6; FIST3; PKY; ANPK; FIST
• UniProt Entry Name: HIPK3_HUMAN

Uniprot Description

HIPK3: homeodomain interacting protein kinase 3 is a CMGC kinase of the DYRK family. Phosphorylates FADD and inhibits fas-mediated Jun NH(2)-terminal kinase activation.

Protein type: Kinase, protein; Nuclear receptor co-regulator; Protein kinase, Ser/Thr (non-receptor); Protein kinase, CMGC; EC 2.7.11.1; CMGC group; DYRK family; HIPK subfamily

Chromosomal Location of Human Ortholog: 11p13

Cellular Component: PML body; cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; ATP binding; protein kinase activity

Biological Process: negative regulation of JNK activity; peptidyl-serine phosphorylation; regulation of transcription, DNA-dependent; apoptosis; peptidyl-threonine phosphorylation; mRNA transcription; protein amino acid phosphorylation; negative regulation of apoptosis