Mouse Hypoxia Inducible Factor 2 Alpha ELISA Kit | HIF-2alpha elisa kit

Mouse HIF-2alpha (Hypoxia Inducible Factor 2 Alpha) ELISA Kit

Cat. Num. AAA2533460

• Gene Names: Epas1; HLF; Hif2a
• Reactivity: Mouse
• Synonyms: Hypoxia Inducible Factor 2 Alpha; Mouse HIF-2alpha (Hypoxia Inducible Factor 2 Alpha) ELISA Kit; HIF-2alpha elisa kit

• Reactivity: Mouse
• Specificity: This kit recognizes Mouse HIF-2alpha in samples. No significant cross-reactivity or interference between Mouse HIF-2alpha and analogues was observed.
• Sequence Length: 874

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.31-20ng/mL
• Sensitivity: 0.19ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Mouse HIF-2alpha were tested 20 times on one plate, respectively.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Mouse HIF-2alpha were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIF-2alpha elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Mouse HIF-2alpha concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse HIF-2alpha. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse HIF-2alpha and Avidin- Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse HIF- 2alpha, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse HIF-2alpha. You can calculate the concentration of Mouse HIF-2alpha in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 8953577
• NCBI GeneID: 29452
• UniProt Accession #: Q9JHS1
• Molecular Weight: 96,718 Da
• NCBI Official Full Name: hypoxia inducible factor 2 alpha
• NCBI Official Synonym Full Names: endothelial PAS domain protein 1
• NCBI Official Symbol: Epas1
• NCBI Official Synonym Symbols: HLF; Hif2a
• NCBI Protein Information: endothelial PAS domain-containing protein 1
• UniProt Protein Name: Endothelial PAS domain-containing protein 1
• UniProt Gene Name: Epas1
• UniProt Synonym Gene Names: Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha
• UniProt Entry Name: EPAS1_RAT

NCBI Description

activates luciferase activity after transfection [RGD, Feb 2006]

Uniprot Description

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD ().