Rat Hypoxia Inducible Factor 2 Alpha (HIF-2alpha) ELISA Kit | HIF-2alpha elisa kit

Rat Hypoxia Inducible Factor 2 Alpha (HIF-2alpha) ELISA Kit

Cat. Num. AAA2601406

• Gene Names: Epas1; HLF; Hif2a
• Reactivity: Rat
• Synonyms: Hypoxia Inducible Factor 2 Alpha (HIF-2alpha); Rat Hypoxia Inducible Factor 2 Alpha (HIF-2alpha) ELISA Kit; Rat Hypoxia Inducible Factor 2 Alpha (HIF-2a) ELISA Kit; HIF-2alpha elisa kit

• Reactivity: Rat
• Specificity: No cross-reaction with other factors.
• Sequence Length: 874

• Samples: Rat serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/ml-0.156ng/ml
• Sensitivity: Up to 0.05ng/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HIF-2alpha elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Rat HIF-2alpha monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 8953577
• NCBI GeneID: 29452
• UniProt Accession #: Q9JHS1
• Molecular Weight: 96,718 Da
• NCBI Official Full Name: hypoxia inducible factor 2 alpha
• NCBI Official Synonym Full Names: endothelial PAS domain protein 1
• NCBI Official Symbol: Epas1
• NCBI Official Synonym Symbols: HLF; Hif2a
• NCBI Protein Information: endothelial PAS domain-containing protein 1; EPAS-1; HIF2 alpha; HIF2-alpha; HIF-2 alpha; HIF-2-alpha; hypoxia inducible factor 2a; hypoxia-inducible factor 2 alpha; hypoxia-inducible factor 2-alpha; hypoxia inducible factor 2, alpha subunit
• UniProt Protein Name: Endothelial PAS domain-containing protein 1
• UniProt Gene Name: Epas1
• UniProt Synonym Gene Names: Hif2a; EPAS-1; HIF-2-alpha; HIF2-alpha
• UniProt Entry Name: EPAS1_RAT

NCBI Description

activates luciferase activity after transfection [RGD, Feb 2006]

Uniprot Description

Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBBP and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD ().