Mouse HGF activator ELISA Kit | HGFAC elisa kit

Mouse Hepatocyte growth factor activator, HGFAC ELISA Kit

Cat. Num. AAA931251

• Gene Names: Hgfac; HGFA
• Reactivity: Mouse
• Synonyms: HGF activator; Mouse Hepatocyte growth factor activator; HGFAC ELISA Kit; Mouse Hepatocyte growth factor activator (HGFAC) ELISA kit; RP11-529E10.2; HGFA; MGC138395; MGC138397; ; HGFAC elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse HGFAC. No significant cross-reactivity or interference between mouse HGFAC and analogues was observed.
• Sequence Length: 653

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of mouse HGFAC is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HGFAC elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HGFAC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HGFAC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HGFAC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HGFAC bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 188219651
• NCBI GeneID: 54426
• NCBI Accession #: NP_062320.2
• NCBI GenBank Nucleotide #: NM_019447.2
• UniProt Accession #: Q9R098; Q9JKV4
• Molecular Weight: 70,568 Da
• NCBI Official Full Name: hepatocyte growth factor activator preproprotein
• NCBI Official Synonym Full Names: hepatocyte growth factor activator
• NCBI Official Symbol: Hgfac
• NCBI Official Synonym Symbols: HGFA
• NCBI Protein Information: hepatocyte growth factor activator; HGF activator
• UniProt Protein Name: Hepatocyte growth factor activator
• Protein Family: Hepatocyte growth factor activator
• UniProt Gene Name: Hgfac
• UniProt Synonym Gene Names: HGF activator; HGFA
• UniProt Entry Name: HGFA_MOUSE

NCBI Description

This gene encodes a serine protease enzyme that proteolytically activates hepatocyte growth factor (HGF) and plays a vital role in the regulation of HGF activity in the regeneration and repair of various tissues. The encoded protein is an inactive zymogen that is proteolytically activated to generate a heterodimeric enzyme consisting of a short chain and a long chain linked by a disulfide bridge. Mice lacking the encoded protein display an impairment in mucosal regeneration after injury. [provided by RefSeq, Jul 2015]

Uniprot Description

HGFAC: Activates hepatocyte growth factor (HGF) by converting it from a single chain to a heterodimeric form. Belongs to the peptidase S1 family.

Protein type: Secreted, signal peptide; EC 3.4.21.-; Protease; Secreted

Cellular Component: extracellular space; rough endoplasmic reticulum; cytoplasm; extracellular region

Molecular Function: peptidase activity; hydrolase activity; serine-type peptidase activity; serine-type endopeptidase activity; catalytic activity

Biological Process: proteolysis