Mouse glutaredoxin 5 ELISA Kit | GLRX5 elisa kit

Mouse Glutaredoxin-related protein 5, mitochondrial, GLRX5 ELISA Kit

Cat. Num. AAA917136

• Gene Names: Glrx5; AU020725; 2310004O13Rik; 2900070E19Rik
• Reactivity: Mouse
• Synonyms: glutaredoxin 5; Mouse Glutaredoxin-related protein 5; mitochondrial; GLRX5 ELISA Kit; mitochondrial (GLRX5) ELISA kit; C14orf87; FLB4739; GRX5; MGC14129; PR01238; PRO1238; glutaredoxin 5 homolog; GLRX5 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse GLRX5. No significant cross-reactivity or interference between mouse GLRX5 and analogues was observed.
• Sequence Length: 152

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 31.25 pg/ml -2000 pg/ml.
• Sensitivity: The minimum detectable dose of mouse GLRX5 is typically less than 7.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GLRX5 elisa kit

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GLRX5 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GLRX5 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GLRX5 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GLRX5 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 21312153
• NCBI GeneID: 73046
• NCBI Accession #: NP_082695.1
• NCBI GenBank Nucleotide #: NM_028419.2
• UniProt Accession #: Q80Y14; Q3YML1; Q9D6E9
• Molecular Weight: 16,292 Da
• NCBI Official Full Name: glutaredoxin-related protein 5, mitochondrial
• NCBI Official Synonym Full Names: glutaredoxin 5 homolog (S. cerevisiae)
• NCBI Official Symbol: Glrx5
• NCBI Official Synonym Symbols: AU020725; 2310004O13Rik; 2900070E19Rik
• NCBI Protein Information: glutaredoxin-related protein 5, mitochondrial; monothiol glutaredoxin-5
• UniProt Protein Name: Glutaredoxin-related protein 5, mitochondrial
• Protein Family: Glutaredoxin-related protein
• UniProt Gene Name: Glrx5
• UniProt Entry Name: GLRX5_MOUSE

Uniprot Description

GLRX5: Monothiol glutaredoxin involved in the biogenesis of iron-sulfur clusters. Required for normal iron homeostasis. Required for normal regulation of hemoglobin synthesis by the iron-sulfur protein ACO1. Defects in GLRX5 are a cause of anemia sideroblastic pyridoxine-refractory autosomal recessive (PRARSA). A form of sideroblastic anemia not responsive to pyridoxine. Sideroblastic anemia is characterized by anemia of varying severity, hypochromic peripheral erythrocytes, systemic iron overload secondary to chronic ineffective erythropoiesis, and the presence of bone marrow ringed sideroblasts. Sideroblasts are characterized by iron-loaded mitochondria clustered around the nucleus. Belongs to the glutaredoxin family. Monothiol subfamily.

Protein type: Oxidoreductase

Cellular Component: mitochondrion; cell soma; dendrite; nucleus

Molecular Function: 2 iron, 2 sulfur cluster binding; electron carrier activity; metal ion binding; iron-sulfur cluster binding; protein disulfide oxidoreductase activity

Biological Process: cell redox homeostasis