Angiopoietin-1 / Ang-1 Active Protein | Ang-1 active protein

Human Angiopoietin-1 / Ang-1

Cat. Num. AAA691590

• Gene Names: ANGPT1; AGP1; AGPT; ANG1
• Reactivity: Human
• Purity: > 95%, by SDS-PAGE and visualized by silver stain
• Synonyms: Angiopoietin-1 / Ang-1; Human Angiopoietin-1 / Ang-1; Recombinant Human Angiopoietin-1; Ang-1 active protein

• Host: HeLa Cells
• Reactivity: Human
• Purity/Purification: > 95%, by SDS-PAGE and visualized by silver stain
• Form/Format: Lyophilized
• Sequence: MSNQRRSPEN SGRRYNRIQH GQCAYTFILP EHDGNCREST TDQYNT NALQ RDAPHVEPDF SSQKLQHLEH MENYTQWLQK LENYIVENMK S EMAQIQQNA VQNHTATMLE IGTSLLSQTA EQTRKLTDVE TQVLNQT SRL EIQLLENSLS TYKLEKQLLQ QTNEILKIHE KNSLLEHKIL EM EGKHKEEL DTLKEEKENL QGLVTRQTYI IQELEKQLNR ATTNNSVL QK QQLELMDTVH NLVNLCTKEG VLLKGGKREE EKPFRDCADV YQA GFNKSGI YTIYINNMPE PKKVFCNMDV NGGGWTVIQH REDGSLDFQ R GWKEYKMGFG NPSGEYWLGN EFIFAITSQR QYMLRIELMD WEGN RAYSQY DRFHIGNEKQ NYRLYLKGHT GTAGKQSSLI LHGADFSTKD ADNDNCMCKC ALMLTGGWWF DACGPSNLNG MFYTAGQNHG KLNGI KWHYF KGPSYSLRST TMMIRPLDFH HHHHH
• Sequence Length: 498

• Endotoxin Level: > 95%, by SDS-PAGE and visualized by silver stain
• Label/Conjugation: His-Tag
• Biological Activity: Determined by the dose-dependent stimulation of the proliferation of human umbilical vein endothelial cells (HUVEC).

Related Product Information for Ang-1 active protein

Human Angiopoietin-1 (Ang-1), a 66 kDa protein consisting of 476 amino acid residues (N21-F496), is fused to a C-terminal His-tag and produced in HeLa cells. The angiopoietin (Ang) family of growth factors includes four members, all of which bind to the endothelial receptor tyrosine kinase Tie2. Two of the Angs, Ang-1 and Ang-4, activate the Tie2 receptor, whereas Ang-2 and Ang-3 inhibit Ang-1-induced Tie2 phosphorylation. Angiopoietin-1 (Ang-1) is a secreted growth factor which binds to and activates the Tie-2 receptor tyrosine kinase. The factor enhances endothelial cell survival and capillary morphogenesis, and also limits capillary permeability. Ang-2 binds the same receptor but fails to activate it: hence, it is a natural inhibitor of Ang-1. Ang-2 destabilizes capillary integrity, facilitating sprouting when ambient vascular endothelial growth factor (VEGF) levels are high, but causing vessel regression when VEGF levels are low. Tie-1 is a Tie-2 homologue but its ligands are unknown. Angiopoietin and Tie genes are expressed in the mammalian metanephros, the precursor of the adult kidney, where they may play a role in endothelial precursor growth. Tie-1-expressing cells can be detected in the metanephros when it first forms and, based on transplantation experiments, these precursors contribute to the generation of glomerular capillaries. During glomerular maturation, podocyte-derived Ang-1 and mesangial-cell-derived Ang-2 may affect growth of nascent capillaries. After birth, vasa rectae acquire their mature configuration and Ang-2 expressed by descending limbs of loops of Henle would be well placed to affect the growth of this medullary microcirculation. Finally, preliminary data implicate angiopoietins in deregulated vessel growth in Wilms\' kidney tumors and in vascular remodeling after nephrotoxicity. Altogether, existing data suggest that VEGF-A and Angiopoietins not only have quite different roles during vascular development, but also very complementary and coordinated roles.

Product Categories/Family for Ang-1 active protein

Cytokines & Growth Factors

NCBI and Uniprot Product Information

• NCBI GI #: 20532340
• NCBI GeneID: 284
• NCBI Accession #: NP_001137.2
• NCBI GenBank Nucleotide #: NM_001146.3
• UniProt Accession #: Q15389; Q5HYA0
• Molecular Weight: 60-70 kDa
• NCBI Official Full Name: angiopoietin-1 isoform 1
• NCBI Official Synonym Full Names: angiopoietin 1
• NCBI Official Symbol: ANGPT1
• NCBI Official Synonym Symbols: AGP1; AGPT; ANG1
• NCBI Protein Information: angiopoietin-1; ANG-1
• UniProt Protein Name: Angiopoietin-1
• UniProt Gene Name: ANGPT1
• UniProt Synonym Gene Names: KIAA0003; ANG-1
• UniProt Entry Name: ANGP1_HUMAN

NCBI Description

Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, Dec 2010]

Uniprot Description

Function: Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Ref.10 Ref.12 Ref.13 Ref.14

Subunit structure: Homooligomer. Interacts with TEK/TIE2. Ref.10 Ref.11 Ref.12

Subcellular location: Secreted.

Post-translational modification: Glycosylated.

Miscellaneous: It may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart.

Sequence similarities: Contains 1 fibrinogen C-terminal domain.

Sequence caution: The sequence BAA02793.2 differs from that shown. Reason: Erroneous initiation.