Sonic Hedgehog Cell Lysate | SHH cell lysate
Mouse Sonic Hedgehog HEK293 Overexpression Lysate
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Samples are stable for up to twelve months from date of receipt.
This Mouse Sonic Hedgehog overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Sonic Hedgehog protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
Uniprot Description
SHH: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. Belongs to the hedgehog family.
Protein type: Cell development/differentiation; Oncoprotein; Cell cycle regulation; Motility/polarity/chemotaxis
Cellular Component: Golgi apparatus; extracellular space; cell surface; endoplasmic reticulum; dendrite; extracellular region; lipid raft; proteinaceous extracellular matrix; transport vesicle; membrane; cell soma; axon; plasma membrane; nucleus
Molecular Function: laminin-1 binding; peptidase activity; protein binding; glycosaminoglycan binding; zinc ion binding; hydrolase activity; metal ion binding; patched binding; calcium ion binding; glycoprotein binding
Biological Process: prostate gland development; skin development; developmental growth; central nervous system development; positive regulation of transcription, DNA-dependent; embryonic skeletal development; mesenchymal cell proliferation; telencephalon regionalization; embryonic foregut morphogenesis; positive regulation of mesenchymal cell proliferation; male genitalia development; anatomical structure formation; oligodendrocyte differentiation; kidney development; neural crest cell migration; inner ear development; embryonic limb morphogenesis; hindbrain development; positive regulation of neuroblast proliferation; tongue morphogenesis; camera-type eye development; cell fate commitment; myotube differentiation; neuron fate commitment; intermediate filament organization; osteoblast development; positive regulation of skeletal muscle cell proliferation; response to ethanol; positive regulation of photoreceptor cell differentiation; regulation of gene expression; positive regulation of cell division; regulation of proteolysis; striated muscle cell differentiation; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; neural tube formation; determination of left/right symmetry; negative regulation of protein catabolic process; negative regulation of apoptosis; embryonic forelimb morphogenesis; limb development; axon guidance; granule cell precursor proliferation; spinal cord dorsal/ventral patterning; positive regulation of striated muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; thalamus development; palate development; signal transduction; Bergmann glial cell differentiation; negative regulation of T cell proliferation; positive regulation of cell proliferation; forebrain development; pancreas development; thyroid gland development; embryonic morphogenesis; heart looping; angiogenesis; vasculogenesis; ventral spinal cord interneuron specification; negative regulation of cell migration; positive thymic T cell selection; positive regulation of granule cell precursor proliferation; gut mesoderm development; intein-mediated protein splicing; regulation of odontogenesis; androgen metabolic process; spinal cord motor neuron differentiation; pattern specification process; odontogenesis of dentine-containing teeth; regulation of cell proliferation; cell proliferation; negative regulation of cell differentiation; stem cell development; embryonic development; dorsal/ventral pattern formation; positive regulation of protein import into nucleus; ureteric bud branching; hindgut morphogenesis; positive regulation of neuron differentiation; lung development; negative regulation of alpha-beta T cell differentiation; negative regulation of Wnt receptor signaling pathway; anatomical structure development; multicellular organismal development; heart development; CD4-positive or CD8-positive, alpha-beta T cell lineage commitment; T cell differentiation in the thymus; lymphoid progenitor cell differentiation; Wnt receptor signaling pathway through beta-catenin; proteolysis; anterior/posterior pattern formation; positive regulation of T cell differentiation in the thymus; cell-cell signaling; hair follicle development; embryonic digestive tract morphogenesis; midbrain development; ectoderm development; positive regulation of smoothened signaling pathway; positive regulation of oligodendrocyte differentiation; oligodendrocyte development; activation of hh target transcription factor; striated muscle development; endocytosis; digestive tract morphogenesis; positive regulation of skeletal muscle development; negative thymic T cell selection; patterning of blood vessels; branching morphogenesis of a tube; polarity specification of anterior/posterior axis; positive regulation of cell differentiation; metanephros development; tongue development; apoptosis; cell fate specification; embryonic hindlimb morphogenesis; odontogenesis; vasculature development; regulation of transcription, DNA-dependent; male genitalia morphogenesis; cell communication; response to axon injury; positive regulation of Wnt receptor signaling pathway; dorsoventral neural tube patterning; organ formation; smoothened signaling pathway; hair follicle morphogenesis; thymus development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; respiratory tube development; positive regulation of immature T cell proliferation in the thymus; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; formation of anatomical boundary; myoblast differentiation; limb bud formation; establishment of cell polarity; neuroblast proliferation; blood coagulation; cell development; positive regulation of alpha-beta T cell differentiation
Research Articles on SHH
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Product Notes
The SHH shh (Catalog #AAA8117043) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 185aa. AAA Biotech's Sonic Hedgehog can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SHH shh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly19 8. It is sometimes possible for the material contained within the vial of "Sonic Hedgehog, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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