PD-L2 Active Protein | PDCD1LG2 active protein

PD-L2 Protein, Cynomolgus, Recombinant (hFc Tag)

Cat. Num. AAA8123589

• Purity: >95% as determined by SDS-PAGE
• Synonyms: PD-L2; PD-L2 Protein; Cynomolgus; Recombinant (hFc Tag); Cynomolgus B7-DC/PD-L2/CD273 Protein (Fc Tag); programmed cell death 1 ligand 2; PDCD1LG2 active protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Pro219

• Species: Cynomolgus
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Leu 20
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the cynomolgus PDCD1LG2(A4GW30)(Met1-Pro219) was expressed with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: Measured by its binding ability in a functional ELISA. Immobilized human PD1-His at 10ug/ml (100uL/well) can bind Cynomolgus PDCD1LG2-Fc. The EC50 of Cynomolgus PDCD1LG2-Fc is 30.0-75.0ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for PDCD1LG2 active protein

Background: Programmed death ligand 2 (PD-L2), also referred to as B7-DC and CD273, is a member of the B7 family of proteins including B7-1, B7-2, B7-H2, B7-H1 (PD-L1), and B7-H3. PD-L2 is a type I membrane protein and structurally consists of an extracellular region containing one V-like and one C-like Ig domain, a transmembrane region, and a short cytoplasmic domain. PD-L2 is expressed on antigen presenting cells, placental endothelium and medullary thymic epithelial cells, and can be induced by LPS in B cells, INF-γ in monocytes, or LPS plus IFN-γ in dendritic cells. The CD28 and B7 protein families are critical regulators of immune responses. PD-L2 and PD-L1 are two ligands for PD-1, member of the CD28/CTLA4 family expressed on activated lymphoid cells, and thus provide signals for regulating T cell activation and immune tolerance. The interaction of B7-DC/PD-1 exhibited a 2-6-fold higher affinity compared with the interaction of B7-H1/PD-1.

Product Categories/Family for PDCD1LG2 active protein

Cancer Drug Targets

References

Latchman Y, et al. (2001) PD-L2 is a second ligand for PD-1 and inhibits T cell activation. Nat Immunol. 2: 261-8.Carreno BM, et al. (2005) Therapeutic opportunities in the B7/CD28 family of ligands and receptors. Curr Opin Pharmacol. 5(4): 424-30.Radhakrishnan S, et al. (2007) B7-DC/PD-L2 cross-linking induces NF-kappaB-dependent protection of dendritic cells from cell death. J Immunol. 178(3): 1426-32.

NCBI and Uniprot Product Information

• UniProt Accession #: A4GW30
• Molecular Weight: The recombinant cynomolgus PDCD1LG2 is a disulfide-linked homodimer. The reduced monomer comprises 441 amino acids and has a calculated molecular mass of 49.6kDa. The apparent molecular mass of the protein is approximately 66kDa respectively in SDS-PAGE.
• Protein Family: Programmed cell death