Canine Nicotinic acetylcholine receptor ELISA Kit | N-AChR elisa kit

Canine Nicotinic acetylcholine receptor ELISA Kit

Cat. Num. AAA743950

• Gene Names: CHRNA4; EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4
• Reactivity: Canine
• Synonyms: Nicotinic acetylcholine receptor; Canine Nicotinic acetylcholine receptor ELISA Kit; N-AChR elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 0.5-10ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This N-ACHR ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine N-ACHR. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for N-AChR elisa kit

Principle of the Assay: N-ACHR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-N-ACHR antibody and an N-ACHR-HRP conjugate. The assay sample and buffer are incubated together with N-ACHR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the N-ACHR concentration since N-ACHR from samples and N-ACHR-HRP conjugate compete for the anti-N-ACHR antibody binding site. Since the number of sites is limited, as more sites are occupied by N-ACHR from the sample, fewer sites are left to bind N-ACHR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The N-ACHR concentration in each sample is interpolated from this standard curve.

Product Categories/Family for N-AChR elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 854159
• NCBI GeneID: 1137
• UniProt Accession #: P43681; Q4JGR7; Q4VAQ5; Q4VAQ6
• Molecular Weight: 60,994 Da
• NCBI Official Full Name: nicotinic acetylcholine receptor, partial
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 4 (neuronal)
• NCBI Official Symbol: CHRNA4
• NCBI Official Synonym Symbols: EBN; BFNC; EBN1; NACHR; NACRA4; NACHRA4
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-4; cholinergic receptor, nicotinic, alpha polypeptide 4; neuronal nicotinic acetylcholine receptor alpha-4 subunit
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-4
• Protein Family: Nicotinic acetylcholine receptor-binding protein
• UniProt Gene Name: CHRNA4
• UniProt Synonym Gene Names: NACRA4
• UniProt Entry Name: ACHA4_HUMAN

NCBI Description

This gene encodes a nicotinic acetylcholine receptor, which belongs to a superfamily of ligand-gated ion channels that play a role in fast signal transmission at synapses. These pentameric receptors can bind acetylcholine, which causes an extensive change in conformation that leads to the opening of an ion-conducting channel across the plasma membrane. This protein is an integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor. Mutations in this gene cause nocturnal frontal lobe epilepsy type 1. Polymorphisms in this gene that provide protection against nicotine addiction have been described. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

nAChRA4: a ligand-gated ion channels that binds acetylcholine and mediates fast signal transmission at synapses. After binding acetylcholine, these pentameric receptors undergo an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. An integral membrane receptor subunit that can interact with either nAChR beta-2 or nAChR beta-4 to form a functional receptor.

Protein type: Channel, ligand-gated; Membrane protein, multi-pass; Membrane protein, integral; Receptor, misc.

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; cell soma; membrane; dendrite; plasma membrane; integral to membrane; cell junction; external side of plasma membrane

Molecular Function: acetylcholine receptor activity; acetylcholine binding; nicotinic acetylcholine-activated cation-selective channel activity; ligand-gated ion channel activity

Biological Process: response to nicotine; regulation of action potential; B cell activation; behavioral response to nicotine; locomotory behavior; respiratory gaseous exchange; sensory perception of pain; signal transduction; DNA repair; regulation of dopamine secretion; synaptic transmission, cholinergic; neurological system process; synaptic transmission; membrane depolarization; regulation of inhibitory postsynaptic membrane potential; regulation of membrane potential; calcium ion transport; response to hypoxia; ion transport; response to oxidative stress; cognition

Disease: Epilepsy, Nocturnal Frontal Lobe, 1; Tobacco Addiction, Susceptibility To