Kallikrein 11 Cell Lysate | KLK11 cell lysate
Human Kallikrein 11 HEK293 Overexpression Lysate
Gene Names
KLK11; TLSP; PRSS20
Applications
Western Blot
Synonyms
Kallikrein 11; Human Kallikrein 11 HEK293 Overexpression Lysate; Human KLK11/Kallikrein-11 HEK293 Cell Lysate (WB positive control); kallikrein-related peptidase 11; Human KLK11 Overexpression Lysate; Human PRSS20 Overexpression Lysate; Human TLSP Overexpression Lysate; KLK11 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
243aa
Sequence
Met1-Asn250
Applicable Applications for KLK11 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human KLK11 isoform 1 (NP_006844.1) (Met 1-Asn 250) with a C-terminal polyhistidine tag was expressed.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for KLK11 cell lysate
kallikrein-related peptidase 11 (KLK11), also known as hippostasin, trypsin-like serine protease, and PRSS2, is a member of the human tissue kallikrein family. It is a subgroup of serine proteases with diverse physiological functions, which are implicated in carcinogenesis and some with potential that serving as novel biomarkers for ovarian and prostate cancer and other diseases. The KLK11 gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Two alternatively spliced forms exist, resulting in 25 (isoform 1) and 282 (isoform 2) amino acid sequences. Isoform 2 is identical to isoform 1, except for an inserted 32 amino acid segment. Isoform 1 is predominantly expressed in the brain whereas isoform 2 is preferentially expressed in the prostate.
This Human Kallikrein 11 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Kallikrein 11 protein from the overexpression lysate was verified.
This Human Kallikrein 11 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Kallikrein 11 protein from the overexpression lysate was verified.
References
Yoshida S., et al., (1998), cDNA cloning and expression of a novel serine protease, TLSP. Biochim. Biophys. Acta 1399:225-228.Mitsui S., et al., (2000), A novel isoform of a kallikrein-like protease, TLSP/hippostasin, (PRSS20), is expressed in the human brain and prostate.Biochem. Biophys. Res. Commun. 272:205-211.Yousef G.M., et al., (2000), Genomic organization, mapping, tissue expression, and hormonal regulation of trypsin-like serine protease (TLSP PRSS20), a new member of the human kallikrein gene family.Genomics 63:88-96.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
30,166 Da
NCBI Official Full Name
kallikrein-11 isoform 1
NCBI Official Synonym Full Names
kallikrein-related peptidase 11
NCBI Official Symbol
KLK11
NCBI Official Synonym Symbols
TLSP; PRSS20
NCBI Protein Information
kallikrein-11; hK11; hippostasin; kallikrein 11; serine protease 20; trypsin-like protease
UniProt Protein Name
Kallikrein-11
Protein Family
UniProt Gene Name
KLK11
UniProt Synonym Gene Names
PRSS20; TLSP; hK11
UniProt Entry Name
KLK11_HUMAN
NCBI Description
Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in multiple transcript variants encoding distinct isoforms which are differentially expressed.[provided by RefSeq, Nov 2009]
Uniprot Description
KLK11: Possible multifunctional protease. Efficiently cleaves 'bz-Phe-Arg-4-methylcoumaryl-7-amide', a kallikrein substrate, and weakly cleaves other substrates for kallikrein and trypsin. Cleaves synthetic peptides after arginine but not lysine residues. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Protease; Secreted; EC 3.4.21.-; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19q13.33
Cellular Component: Golgi apparatus; extracellular space
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis
Research Articles on KLK11
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Product Notes
The KLK11 klk11 (Catalog #AAA8114115) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 243aa. AAA Biotech's Kallikrein 11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the KLK11 klk11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asn25 0. It is sometimes possible for the material contained within the vial of "Kallikrein 11, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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