Canine Pancreatic Polypeptide ELISA Kit | PP elisa kit

Canine Pancreatic Polypeptide ELISA Kit

Cat. Num. AAA741715

• Gene Names: PPY; PNP
• Reactivity: Canine
• Synonyms: Pancreatic Polypeptide; Canine Pancreatic Polypeptide ELISA Kit; PP elisa kit

• Reactivity: Canine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 25-500pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This PP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Canine PP. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PP elisa kit

Principle of the Assay: PP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PP antibody and an PP-HRP conjugate. The assay sample and buffer are incubated together with PP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PP concentration since PP from samples and PP-HRP conjugate compete for the anti-PP antibody binding site. Since the number of sites is limited, as more sites are occupied by PP from the sample, fewer sites are left to bind PP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PP concentration in each sample is interpolated from this standard curve.

Product Categories/Family for PP elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 119572034
• NCBI GeneID: 5539
• Molecular Weight: 10,445 Da
• NCBI Official Full Name: pancreatic polypeptide
• NCBI Official Synonym Full Names: pancreatic polypeptide
• NCBI Official Symbol: PPY
• NCBI Official Synonym Symbols: PNP
• NCBI Protein Information: pancreatic prohormone; PP; pancreatic polypeptide Y; prepro-PP (prepropancreatic polypeptide)
• UniProt Protein Name: Pancreatic prohormone
• Protein Family: Pancreatic polypeptide
• UniProt Gene Name: PPY
• UniProt Synonym Gene Names: PNP; PP; PH; PI
• UniProt Entry Name: PAHO_HUMAN

NCBI Description

This gene belongs to the NPY family and it encodes a protein that is synthesized as a 95 aa polypeptide precursor in the pancreatic islets of Langerhans. It is cleaved into two peptide products; the active hormone of 36 aa and an icosapeptide of unknown function. The hormone acts as a regulator of pancreatic and gastrointestinal functions and may be important in the regulation of food intake. Plasma level of this hormone has been shown to be reduced in conditions associated with increased food intake and elevated in anorexia nervosa. In addition, infusion of this hormone in obese rodents has shown to decrease weight gain. [provided by RefSeq, Jul 2008]

Uniprot Description

PPY: Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions. Belongs to the NPY family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; extracellular region

Molecular Function: neuropeptide hormone activity; G-protein-coupled receptor binding; hormone activity; receptor binding

Biological Process: cell-cell signaling; neuropeptide signaling pathway; digestion; regulation of appetite; protein secretion; feeding behavior