KIR2DL5 Cell Lysate | KIR2DL5A cell lysate
Human KIR2DL5 HEK293 Overexpression Lysate
Gene Names
KIR2DL5A; CD158F; KIR2DL5; KIR2DL5B; KIR2DL5.1; KIR2DL5.3
Applications
Western Blot
Synonyms
KIR2DL5; Human KIR2DL5 HEK293 Overexpression Lysate; Human KIR2DL5A HEK293 Cell Lysate (WB positive control); killer cell immunoglobulin like receptor; two Ig domains and long cytoplasmic tail 5A; Human CD158F Overexpression Lysate; Human KIR2DL5 Overexpression Lysate; Human KIR2DL5.1 Overexpression Lysate; Human KIR2DL5.3 Overexpression Lysate; KIR2DL5A cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
457aa
Sequence
Met1-His240
Applicable Applications for KIR2DL5A cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human KIR2DL5A (NP_065396.1) (Met1-His240) was expressed with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for KIR2DL5A cell lysate
KIR2DL5A (Killer Cell Immunoglobulin Like Receptor, Two Ig Domains And Long Cytoplasmic Tail 5A) is a Protein Coding gene. The encoded protein belongs to the immunoglobulin superfamily. The most recently described member of the KIR family, KIR2DL5, is represented in human populations by at least four gene variants, whose exons differ by two to eight nucleotides. KIR2DL5 (CD158f) is the most recently identified inhibitory member of human killer-cell Ig-like receptors (KIRs), which enable NK cells to sense self-HLA. KIR2DL5 is encoded by two paralogous genes displaying copy number variation and allelic polymorphism-KIR2DL5A and KIR2DL5B. Functional polymorphisms in both its promoter and its coding regions are critical for understanding the KIR2DL5 role in immunity and its importance for human health.
This Human KIR2DL5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of KIR2DL5 protein from the overexpression lysate was verified.
This Human KIR2DL5 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of KIR2DL5 protein from the overexpression lysate was verified.
Product Categories/Family for KIR2DL5A cell lysate
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40,678 Da
NCBI Official Full Name
killer cell immunoglobulin-like receptor 2DL5A
NCBI Official Synonym Full Names
killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5A
NCBI Official Symbol
KIR2DL5A
NCBI Official Synonym Symbols
CD158F; KIR2DL5; KIR2DL5B; KIR2DL5.1; KIR2DL5.3
NCBI Protein Information
killer cell immunoglobulin-like receptor 2DL5A; killer-cell Ig-like receptor; killer cell immunoglobulin-like receptor KIR2DL5A; killer cell immunoglobulin-like receptor, two domains, long cytoplasmic tail, 5
UniProt Protein Name
Killer cell immunoglobulin-like receptor 2DL5A
Protein Family
UniProt Gene Name
KIR2DL5A
UniProt Synonym Gene Names
CD158F; CD158F1; KIR2DL5
NCBI Description
Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. [provided by RefSeq, Jul 2008]
Uniprot Description
Receptor on natural killer (NK) cells for HLA-C alleles. Inhibits the activity of NK cells thus preventing cell lysis.
Research Articles on KIR2DL5A
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Product Notes
The KIR2DL5A kir2dl5a (Catalog #AAA8115626) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 457aa. AAA Biotech's KIR2DL5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the KIR2DL5A kir2dl5a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-His24 0. It is sometimes possible for the material contained within the vial of "KIR2DL5, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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