A33 Cell Lysate | GPA33 cell lysate
Mouse A33 HEK293 Overexpression Lysate
Gene Names
Gpa33; BB116197; 2010310L10Rik; 2210401D16Rik
Applications
Western Blot
Synonyms
A33; Mouse A33 HEK293 Overexpression Lysate; Mouse GPA33/Glycoprotein A33 HEK293 Cell Lysate (WB positive control); glycoprotein A33 (transmembrane); Mouse 2010310L10Rik Overexpression Lysate; Mouse 2210401D16Rik Overexpression Lysate; Mouse BB116197 Overexpression Lysate; GPA33 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
225aa
Sequence
Met1-Ile235
Applicable Applications for GPA33 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse GPA33 (NP_067623.1) extracellular domain (Met 1-Ile 235) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for GPA33 cell lysate
Cell surface A33 antigen, also known as glycoprotein A33, is a single-pass type I membrane protein that is expressed in the normal gastrointestinal epithelium and 95% of colon cancers. GPA33 contains one Ig-like C2-type (immunoglobulin-like) domain and one Ig-like V-type (immunoglobulin-like) domain. The open reading frame encodes a 319-amino acid polypeptide having a putative secretory signal sequence and 3 potential glycosylation sites. The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. Intracellular traffic and recycling to the cell surface appear to play a major role in GPA33 function and to have an influence on its surface density superseding translational regulation. GPA33 has become a promising target of immunologic therapy strategies, but its biologic function and potential role in tumorigenesis are unknown. EpCAM protein and GPA33 mRNA expressions are specific and sensitive markers of Barrett's metaplasia (BM). GPA33 may also play a role in cell-cell recognition and signaling.
This Mouse A33 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of A33 protein from the overexpression lysate was verified.
This Mouse A33 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of A33 protein from the overexpression lysate was verified.
Product Categories/Family for GPA33 cell lysate
References
Heath J.K., et al., 1997, Proc. Natl. Acad. Sci. USA. 94:469-74. Ritter G., et al., 1997, Biochem. Biophys. Res. Commun. 236:682-6. Frey, D. et al., 2008, Cancer Biother Radiopharm 23 (1):65-73. Rageul, J. et al., 2009, Int J Cancer 125 (12):2802-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
35,692 Da
NCBI Official Full Name
cell surface A33 antigen
NCBI Official Synonym Full Names
glycoprotein A33 (transmembrane)
NCBI Official Symbol
Gpa33
NCBI Official Synonym Symbols
BB116197; 2010310L10Rik; 2210401D16Rik
NCBI Protein Information
cell surface A33 antigen
UniProt Protein Name
Cell surface A33 antigen
Protein Family
UniProt Gene Name
Gpa33
UniProt Synonym Gene Names
mA33
Uniprot Description
May play a role in cell-cell recognition and signaling.
Research Articles on GPA33
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Product Notes
The GPA33 gpa33 (Catalog #AAA8116692) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 225aa. AAA Biotech's A33 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the GPA33 gpa33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ile23 5. It is sometimes possible for the material contained within the vial of "A33, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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