Transferrin Receptor/TFRC Cell Lysate | TFRC cell lysate
Mouse Transferrin Receptor/TFRC HEK293 Overexpression Lysate
Gene Names
Tfrc; TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; Mtvr-1; AI195355; AI426448; AU015758; 2610028K12Rik; E430033M20Rik
Applications
Western Blot
Synonyms
Transferrin Receptor/TFRC; Mouse Transferrin Receptor/TFRC HEK293 Overexpression Lysate; Mouse Transferrin Receptor/TFRC/CD71 HEK293 Cell Lysate (WB positive control); transferrin receptor; Mouse 2610028K12Rik Overexpression Lysate; Mouse AI195355 Overexpression Lysate; Mouse AI426448 Overexpression Lysate; Mouse AU015758 Overexpression Lysate; Mouse CD71 Overexpression Lysate; Mouse E430033M20Rik Overexpression Lysate; Mouse Mtvr-1 Overexpression Lysate; Mouse Mtvr1 Overexpression Lysate; Mouse p90 Overexpression Lysate; Mouse TFR Overexpression Lysate; Mouse TFR1 Overexpression Lysate; Mouse TR Overexpression Lysate; Mouse Trfr Overexpression Lysate; TFRC cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
694aa
Sequence
Cys89-Phe763
Applicable Applications for TFRC cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain of mouse TFRC (Q62351) (Cys 89-Phe 763) was fused with a polyhistidine tag at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TFRC cell lysate
Transferrin receptor protein 1, also known as transferrin receptor, Trfr, p9, CD71 and TFRC, is a single-pass type II membrane protein that belongs to the peptidase M28 family and M28B subfamily. TFRC/CD71 is a membrane-bound protein expressed in larger amounts in proliferating. The specific expression of TFRC can represent a tool or a therapeutic target in solid tumours expressing this antigen. Transferrin receptor is necessary for development of erythrocytes and the nervous system. TFRC/CD71 is regulated by cellular iron levels through binding of the iron regulatory proteins, IRP1 and IRP2, to iron-responsive elements in the 3'-UTR. Up-regulated upon mitogenic stimulation. TFRC/CD71 represents a marker of malignant transformation in the pancreas that could be applied as potential and therapeutic target.
This Mouse Transferrin Receptor/TFRC overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Transferrin Receptor/TFRC protein from the overexpression lysate was verified.
This Mouse Transferrin Receptor/TFRC overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Transferrin Receptor/TFRC protein from the overexpression lysate was verified.
References
Douabin-Gicquel V., et al., 2001, Hum. Genet. 109:393-401. Ryschich, E. et al., 2004, Eur J Cancer. 40 (9):1418-22. Tosoni D., et al., 2005, Cell 123:875-888. Wollscheid B., et al., 2009, Nat. Biotechnol. 27:378-386.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
transferrin receptor protein 1
NCBI Official Synonym Full Names
transferrin receptor
NCBI Official Symbol
Tfrc
NCBI Official Synonym Symbols
TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; Mtvr-1; AI195355; AI426448; AU015758; 2610028K12Rik; E430033M20Rik
NCBI Protein Information
transferrin receptor protein 1; OTTMUSP00000033382; mammary tumor virus receptor 1
UniProt Protein Name
Transferrin receptor protein 1
Protein Family
UniProt Entry Name
TFR1_MOUSE
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Product Notes
The TFRC (Catalog #AAA8116847) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 694aa. AAA Biotech's Transferrin Receptor/TFRC can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TFRC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Cys89-Phe7 63. It is sometimes possible for the material contained within the vial of "Transferrin Receptor/TFRC, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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