SR-BI/SCARB1 Cell Lysate | SCARB1 cell lysate
Human SR-BI/SCARB1 HEK293 Overexpression Lysate
Gene Names
SCARB1; CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
Applications
Western Blot
Synonyms
SR-BI/SCARB1; Human SR-BI/SCARB1 HEK293 Overexpression Lysate; Human SCARB1/CD36L1/CLA-1 HEK293 Cell Lysate (WB positive control); scavenger receptor class B; member 1; Human CD36L1 Overexpression Lysate; Human CLA-1 Overexpression Lysate; Human CLA1 Overexpression Lysate; Human HDLQTL6 Overexpression Lysate; Human SR-BI Overexpression Lysate; Human SRB1 Overexpression Lysate; SCARB1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
422aa
Sequence
Pro33-Tyr443
Applicable Applications for SCARB1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mature form of human SCARB1 (NP_005496.4) extracellular domain (Pro 33-Tyr 443) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SCARB1 cell lysate
Scavenger receptor class B, member 1 (SCARB1), also known as CD36L1, is a member of the scavenger receptor family. SCARB1 is expressed primarily in liver and non placental steroidogenic tissues, and predominantly localized to cholesterol and sphingomyelin-enriched domains within the plasma membrane. SCARB1 is proposed as a receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells, and is involved in a wide variety of physilogical processes. As a key component in the reverse cholesterol transport pathway, SCARB1 binds high density lipoproteins (HDLs) and mediates selective cholesterol uptake by a mechanism distinct from the LDL pathway. High density lipoproteins (HDLs) play a critical role in cholesterol metabolism and their plasma concentrations are inversely correlated with risk for atherosclerosis. SCARB1 may thus serve as a useful marker that predicts variation in baseline lipid levels and postprandial lipid response. The mouse SCARB1 has been shown to exert actions in determining the levels of plasma lipoprotein cholesterol and the accumulation of cholesterol stores in the adrenal gland.
This Human SR-BI/SCARB1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SR-BI/SCARB1 protein from the overexpression lysate was verified.
This Human SR-BI/SCARB1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SR-BI/SCARB1 protein from the overexpression lysate was verified.
References
Murao, K. et al., 1997, J. Biol. Chem. 272(28): 17551-17557. Ikemoto, M. et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97 (12): 6538-6543. Husemann, J. et al., 2001, Am. J. Pathol. 158 (3): 825-832. Williams, D.L. et al., 2001, Endocr. Res. 26 (4): 639-651. Bulte, B. S. et al., 2002, J. Biol. Chem. 277 (39): 36092-36099. Duncan, K.G. et al., 2002, Biochem. Biophys. Res. Commun. 292 (4): 1017-1022.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
56,170 Da
NCBI Official Full Name
scavenger receptor class B member 1 isoform 1
NCBI Official Synonym Full Names
scavenger receptor class B, member 1
NCBI Official Symbol
SCARB1
NCBI Official Synonym Symbols
CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6
NCBI Protein Information
scavenger receptor class B member 1; CD36 and LIMPII analogous 1; CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1; scavenger receptor class B type III
Protein Family
NCBI Description
The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]
Research Articles on SCARB1
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Product Notes
The SCARB1 (Catalog #AAA8114386) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 422aa. AAA Biotech's SR-BI/SCARB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SCARB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Pro33-Tyr4 43. It is sometimes possible for the material contained within the vial of "SR-BI/SCARB1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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