SPINK4 cell lysate
Human SPINK4 HEK293 Overexpression Lysate
Gene Names
SPINK4; PEC60; HEL136; PEC-60
Applications
Western Blot
Synonyms
SPINK4; Human SPINK4 HEK293 Overexpression Lysate; Human SPINK4 HEK293 Cell Lysate (WB positive control); serine peptidase inhibitor; Kazal type 4; Human HEL136 Overexpression Lysate; Human MGC133107 Overexpression Lysate; Human PEC-60 Overexpression Lysate; Human PEC60 Overexpression Lysate; Human SPINK4 Overexpression Lysate; SPINK4 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
71aa
Sequence
Met1-Cys86
Applicable Applications for SPINK4 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human SPINK4 (NP_055286.1) (Met 1-Cys 86) was expressed, fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SPINK4 cell lysate
Serine protease inhibitor Kazal-type 4, also known as Peptide PEC-6 homolog and SPINK4, is a secreted protein that contains one Kazal-like domain. SPINK4 is a member of the SPINK protein family. The gene family of serine protease inhibitors of the Kazal type (SPINK) are functional and positional candidate genes for celiac disease (CD). SPINK1 plays an important role in protecting the pancreas against excessive trypsinogen activation. It is a potent natural inhibitor of pancreatic trypsin activity. SPINK1 mutations are associated with the development of acute and chronic pancreatitis and have been detected in all forms of chronic pancreatitis. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged infertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK9 was identified in human skin. Its expression was strong in palmar epidermis, but not detectable or very low in non palmoplantar skin.
This Human SPINK4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SPINK4 protein from the overexpression lysate was verified.
This Human SPINK4 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SPINK4 protein from the overexpression lysate was verified.
References
Schneider, A. et al., 2004, Gastroenterol Clin North Am. 33 (4): 789-806. Wapenaar, MC. et al., 2007, Immunogenetics. 59 (5): 349-57. Brattsand, M. et al., 2009, J Invest Dermatol. 129 (7): 1656-65. Chen, T. et al., 2009, Proteins. 77 (1): 209-19. Noah, TK. et al., 2010, Exp Cell Res. 316 (3): 452-65.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
9,454 Da
NCBI Official Full Name
serine protease inhibitor Kazal-type 4
NCBI Official Synonym Full Names
serine peptidase inhibitor, Kazal type 4
NCBI Official Symbol
SPINK4
NCBI Official Synonym Symbols
PEC60; HEL136; PEC-60
NCBI Protein Information
serine protease inhibitor Kazal-type 4; peptide PEC-60 homolog; gastrointestinal peptide; epididymis luminal protein 136; serine protease inhibitor, Kazal type 4
UniProt Protein Name
Serine protease inhibitor Kazal-type 4
Protein Family
UniProt Gene Name
SPINK4
UniProt Entry Name
ISK4_HUMAN
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Product Notes
The SPINK4 spink4 (Catalog #AAA8114640) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 71aa. AAA Biotech's SPINK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SPINK4 spink4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Cys86. It is sometimes possible for the material contained within the vial of "SPINK4, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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