SPINK4 elisa pair set

SPINK4 Matched ELISA Antibody Pair Set, Human

Cat. Num. AAA8118392

• Synonyms: SPINK4; SPINK4 Matched ELISA Antibody Pair Set; Human; Human SPINK4 ELISA Pair Set; serine peptidase inhibitor; Kazal type 4; HEL136 Matched ELISA Antibody Pair Set; MGC133107 Matched ELISA Antibody Pair Set; PEC-60 Matched ELISA Antibody Pair Set; PEC60 Matched ELISA Antibody Pair Set; SPINK4 elisa pair set

• Specificity: Quantitative determination of Human SPINK4

• Assay Type: Solid Phase Sandwich ELISA
• Detection Range: 19.53-1250pg/mL
• Sensitivity: 19.53pg/ml
• Materials Provided: 1. Capture Antibody 1 mg/mL of rabbit anti-Human SPINK4 monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2ug/mL in PBS before coating. ; 2. Detection Antibody 0.2 mg/mL of rabbit anti-Human SPINK4 monoclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-Protector, pH 7.4, store at 4 degree C). Dilute to working concentration of 0.5ug/mL in detection antibody dilution buffer before use. ; 3. Standard Each vial contains 135 ng of recombinant Human SPINK4. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 degree C to -80 degree C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1250 pg/mL is recommended.
• Preparation and Storage: Capture Antibody: Aliquot and store at -20 degree C to -80 degree C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.; Detection Antibody: Store at 4 degree C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!; Standard: Store lyophilized standard at -20 degree C to -80 degree C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80 degree C for up to 1 month. Avoid repeated freeze-thaw cycles.

Testing Data

Related Product Information for SPINK4 elisa pair set

Serine protease inhibitor Kazal-type 4, also known as Peptide PEC-6 homolog and SPINK4, is a secreted protein that contains one Kazal-like domain. SPINK4 is a member of the SPINK protein family. The gene family of serine protease inhibitors of the Kazal type (SPINK) are functional and positional candidate genes for celiac disease (CD). SPINK1 plays an important role in protecting the pancreas against excessive trypsinogen activation. It is a potent natural inhibitor of pancreatic trypsin activity. SPINK1 mutations are associated with the development of acute and chronic pancreatitis and have been detected in all forms of chronic pancreatitis. SPINK2 functions as a trypsin/acrosin inhibitor and is synthesized mainly in the testis and seminal vesicle where its activity is engaged infertility. The SPINK2 protein contains a typical Kazal domain composed by six cysteine residues forming three disulfide bridges. SPINK9 was identified in human skin. Its expression was strong in palmar epidermis, but not detectable or very low in non palmoplantar skin.

This SPINK4 Matched ELISA Antibody Pair Set, Human is a solid phase sandwich ELISA for quantitative determination of Human SPINK4. It contains Human SPINK4 capture antibody, Human SPINK4 detector antibody (HRP) and a highly purified HEK293-expressed recombinant Human SPINK4 protein.

References

Schneider, A. et al., 2004, Gastroenterol Clin North Am. 33 (4): 789-806. Wapenaar, MC. et al., 2007, Immunogenetics. 59 (5): 349-57. Brattsand, M. et al., 2009, J Invest Dermatol. 129 (7): 1656-65. Chen, T. et al., 2009, Proteins. 77 (1): 209-19. Noah, TK. et al., 2010, Exp Cell Res. 316 (3): 452-65.