SLAMF6 cell lysate
Human SLAMF6 HEK293 Overexpression Lysate
Gene Names
SLAMF6; KALI; NTBA; CD352; KALIb; Ly108; NTB-A; SF2000
Applications
Western Blot
Synonyms
SLAMF6; Human SLAMF6 HEK293 Overexpression Lysate; Human SLAMF6/Ly108 HEK293 Cell Lysate (WB positive control); SLAM family member 6; Human CD352 Overexpression Lysate; Human KALI Overexpression Lysate; Human KALIb Overexpression Lysate; Human Ly108 Overexpression Lysate; Human NTB-A Overexpression Lysate; Human NTBA Overexpression Lysate; Human SF2000 Overexpression Lysate; SLAMF6 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
212aa
Sequence
Met1-Met226
Applicable Applications for SLAMF6 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human SLAMF6 (Q96DU3-1) (Met1-Met226) was expressed with six amino acids (LEVLFQ) at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SLAMF6 cell lysate
SLAM family member 6, also known as Activating NK receptor, NK-T-B-antigen, NTB-A, SLAMF6, KALI and Ly18, is a single-pass type I membrane protein that belongs to the CD2 subfamily of the immunoglobulin superfamily. SLAMF6/Ly18 contains one Ig-like (immunoglobulin-like) domain. It is expressed by all (resting and activated) natural killer cells (NK), T-and B-lymphocytes. SLAMF6/Ly18 triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. SLAMF6/Ly18 is a homodimer. It interacts with PTN6 and, upon phosphorylation, with PTN11 and SH2D1A/SAP. SLAMF6/Ly18 undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It may function as a coreceptor in the process of NK cell activation. SLAMF6/Ly18 can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients.
This Human SLAMF6 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLAMF6 protein from the overexpression lysate was verified.
This Human SLAMF6 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SLAMF6 protein from the overexpression lysate was verified.
Product Categories/Family for SLAMF6 cell lysate
References
Gray CW. et al., 2000, Eur J Biochem. 267 (18): 5699-710. Bottino C. et al., 2001, J Exp Med. 194 (3): 235-46. Valdez PA. et al., 2004, J Biol Chem. 279 (18): 18662-9. Claus M. et al., 2007, Front Biosci. 13: 956-65.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
24,976 Da
NCBI Official Full Name
SLAM family member 6 isoform 1
NCBI Official Synonym Full Names
SLAM family member 6
NCBI Official Symbol
SLAMF6
NCBI Official Synonym Symbols
KALI; NTBA; CD352; KALIb; Ly108; NTB-A; SF2000
NCBI Protein Information
SLAM family member 6; NK-T-B-antigen; NTBA receptor; activating NK receptor; natural killer-, T- and B-cell antigen
UniProt Protein Name
SLAM family member 6
Protein Family
UniProt Gene Name
SLAMF6
UniProt Synonym Gene Names
KALI; NTB-A
UniProt Entry Name
SLAF6_HUMAN
NCBI Description
The protein encoded by this gene is a type I transmembrane protein, belonging to the CD2 subfamily of the immunoglobulin superfamily. This encoded protein is expressed on Natural killer (NK), T, and B lymphocytes. It undergoes tyrosine phosphorylation and associates with the Src homology 2 domain-containing protein (SH2D1A) as well as with SH2 domain-containing phosphatases (SHPs). It functions as a coreceptor in the process of NK cell activation. It can also mediate inhibitory signals in NK cells from X-linked lymphoproliferative patients. Alternative splicing results in multiple transcript variants encoding distinct isoforms.[provided by RefSeq, May 2010]
Uniprot Description
SLAMF6: Triggers cytolytic activity only in natural killer cells (NK) expressing high surface densities of natural cytotoxicity receptors. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, misc.
Chromosomal Location of Human Ortholog: 1q23.2
Cellular Component: integral to membrane; plasma membrane
Molecular Function: receptor activity
Research Articles on SLAMF6
Similar Products
Product Notes
The SLAMF6 slamf6 (Catalog #AAA8114836) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 212aa. AAA Biotech's SLAMF6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SLAMF6 slamf6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Met22 6. It is sometimes possible for the material contained within the vial of "SLAMF6, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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