SEMA6A cell lysate
Human SEMA6A HEK293 Overexpression Lysate
Gene Names
SEMA6A; VIA; SEMA; HT018; SEMAQ; SEMA6A1
Applications
Western Blot
Synonyms
SEMA6A; Human SEMA6A HEK293 Overexpression Lysate; Human Semaphorin 6A/SEMA6A HEK293 Cell Lysate (WB positive control); sema domain; transmembrane domain (TM); and cytoplasmic domain; (semaphorin) 6A; Human HT018 Overexpression Lysate; Human SEMA Overexpression Lysate; Human SEMA6A1 Overexpression Lysate; Human SEMAQ Overexpression Lysate; Human VIA Overexpression Lysate; SEMA6A cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
642aa
Sequence
Met1-Thr649
Applicable Applications for SEMA6A cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human SEMA6A (NP_065847.1) extracellular domain (Met 1-Thr 649) was expressed, with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for SEMA6A cell lysate
SEMA6A, the first identified class 6 semaphorin, is contributed to regulate vascular development and adult angiogenesis. SEMA6A could inhibit proliferation, migration, and invasion in different glioma cell lines. That SEMA6A may be a potential prognostic biomarker in the treatment of GBM. Among different semaphorins tested by reverse transcriptase-polymerase chain reaction in human immune cells, the expression of class 6 transmembrane semaphorin SEMA6A was restricted to dendritic cells (DCs).
This Human SEMA6A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SEMA6A protein from the overexpression lysate was verified.
This Human SEMA6A overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of SEMA6A protein from the overexpression lysate was verified.
References
Klostermann A., et al., 2000, J. Biol. Chem. 275:39647-53. Nagase T., et al., 2000, DNA Res. 7:65-73. Schmutz J., et al., 2004, Nature 431:268-74. Neufeld, G. et al., 2005, Front Biosci. 10 : 751-60. Fiore, R. et al., 2005, Mol Cell Biol. 25 (6):2310-9.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
116,336 Da
NCBI Official Full Name
semaphorin-6A isoform 2
NCBI Official Synonym Full Names
semaphorin 6A
NCBI Official Symbol
SEMA6A
NCBI Official Synonym Symbols
VIA; SEMA; HT018; SEMAQ; SEMA6A1
NCBI Protein Information
semaphorin-6A
UniProt Protein Name
Semaphorin-6A
Protein Family
UniProt Gene Name
SEMA6A
UniProt Synonym Gene Names
KIAA1368; SEMAQ; Sema VIA; SEMA6A-1
NCBI Description
The transmembrane semaphorin SEMA6A is expressed in developing neural tissue and is required for proper development of the thalamocortical projection (Leighton et al., 2001 [PubMed 11242070]).[supplied by OMIM, Feb 2011]
Uniprot Description
Cell surface receptor for PLXNA2 that plays an important role in cell-cell signaling. Required for normal granule cell migration in the developing cerebellum. Promotes reorganization of the actin cytoskeleton and plays an important role in axon guidance in the developing central nervous system. Can act as repulsive axon guidance cue. Has repulsive action towards migrating granular neurons. May play a role in channeling sympathetic axons into the sympathetic chains and controlling the temporal sequence of sympathetic target innervation ().
Research Articles on SEMA6A
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Product Notes
The SEMA6A sema6a (Catalog #AAA8114463) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 642aa. AAA Biotech's SEMA6A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the SEMA6A sema6a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Thr64 9. It is sometimes possible for the material contained within the vial of "SEMA6A, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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