OSTM1 cell lysate
Human OSTM1 HEK293 Overexpression Lysate
Gene Names
OSTM1; GL; GIPN; OPTB5; HSPC019
Applications
Western Blot
Synonyms
OSTM1; Human OSTM1 HEK293 Overexpression Lysate; Human OSTM1 HEK293 Cell Lysate (WB positive control); osteopetrosis associated transmembrane protein 1; Human GIPN Overexpression Lysate; Human GL Overexpression Lysate; Human HSPC019 Overexpression Lysate; Human OPTB5 Overexpression Lysate; OSTM1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
264aa
Sequence
Met1-Pro284
Applicable Applications for OSTM1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain of human OSTM1 (NP_054747.2) (Met 1-Pro 284) was expressed, fused with a C-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for OSTM1 cell lysate
Osteopetrosis-associated transmembrane protein 1 (OSTM1) is a Single-pass type I membrane protein. It is expressed in many hematopoietic cells of the myeloid and lymphoid B-and T-lineages. The analysis of OSTM1 association with CLCN7 demonstrated that OSTM1 requires CLCN7 to localize to lysosomes, whereas the formation of a CLCN7-OSTM1 complex is required to stabilize CLCN7. OSTM1 plays a major role in myelopoiesis and lymphopoiesis and provided evidence of a crosstalk mechanism between hematopoietic cells for osteoclast activation. Thus, OSTM1 has an important role in osteoclast function and activation. The loss of function of OSTM1 results in deregulation of multiple hematopoietic lineages in addition to osteoclast lineage, OSTM1-defect patients display the most severe recessive osteopetrosis phenotype and die at early ages. Furthermore, it is suggested that OSTM1 has a primary role in neural development not related to lysosomal dysfunction. The canonical Wnt/beta-catenin signaling pathway may be a molecular basis for OSTM1 mutations and severe autosomal recessive osteopetrosis (ARO).
This Human OSTM1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of OSTM1 protein from the overexpression lysate was verified.
This Human OSTM1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of OSTM1 protein from the overexpression lysate was verified.
References
1. Chalhoub, N. et al., 2003, Nat Med. 9 (4): 399-406. 2. Quarello, P. et al., 2004, J Bone Miner Res. 19 (7): 1194-1199. 3. Lange, PF. et al., 2006, Nature. 440 (7081): 220-223 4. Maranda, B. et al., 2008, J Bone Miner Res. 23 (2): 296-300. 5. Feigin, ME.et al., 2008, Cell Signal. 20 (5): 949-957. 6. Pata, M.et al., 2008, J Biol Chem. 283 (45): 30522-30530.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
37,257 Da
NCBI Official Full Name
osteopetrosis-associated transmembrane protein 1
NCBI Official Synonym Full Names
osteopetrosis associated transmembrane protein 1
NCBI Official Symbol
OSTM1
NCBI Official Synonym Symbols
GL; GIPN; OPTB5; HSPC019
NCBI Protein Information
osteopetrosis-associated transmembrane protein 1
UniProt Protein Name
Osteopetrosis-associated transmembrane protein 1
Protein Family
UniProt Gene Name
OSTM1
UniProt Synonym Gene Names
GL
UniProt Entry Name
OSTM1_HUMAN
NCBI Description
This gene encodes a protein that may be involved in the degradation of G proteins via the ubiquitin-dependent proteasome pathway. The encoded protein binds to members of subfamily A of the regulator of the G-protein signaling (RGS) family through an N-terminal leucine-rich region. This protein also has a central RING finger-like domain and E3 ubiquitin ligase activity. This protein is highly conserved from flies to humans. Defects in this gene may cause the autosomal recessive, infantile malignant form of osteopetrosis. [provided by RefSeq, Jul 2008]
Research Articles on OSTM1
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Product Notes
The OSTM1 ostm1 (Catalog #AAA8114229) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 264aa. AAA Biotech's OSTM1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the OSTM1 ostm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Pro28 4. It is sometimes possible for the material contained within the vial of "OSTM1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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