Rabbit Synapsin 1 Polyclonal Antibody | anti-SYN1 antibody

Anti-Synapsin 1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100361

• Gene Names: SYN1; SYNI; SYN1a; SYN1b
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot, Immunoprecipitation
• Purity: Protein A & Antigen Affinity
• Synonyms: Synapsin 1; Polyclonal Antibody; Anti-Synapsin 1 Antibody; Rabbit Polyclonal; Synapsin I/Synapsin-1 Antibody; Rabbit PAb; Antigen Affinity Purified; synapsin I; Anti-RP1-230G1.2 Antibody; Anti-SYN1a Antibody; Anti-SYN1b Antibody; Anti-SYNI Antibody; anti-SYN1 antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Synapsin 1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SYN1 antibody: Western Blot (WB), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:2000; IP: 4-6uL/mg of lysate
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human SYN1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human SYN1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunoprecipitation (IP)

(SYN1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg A549 Whole Cell Lysate2 uL anti-SYN1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-SYN1 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 74 kDaObserved band size: 105 kDa)

Western Blot (WB)

(Anti-SYN1 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse brain tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:74 kDaObserved band size:74 kDa)

Related Product Information for anti-SYN1 antibody

Synapsin I (SynI) is a synaptic vesicle (SV) phosphoprotein playing multiple roles in synaptic transmission and plasticity by differentially affecting crucial steps of SV trafficking in excitatory and inhibitory synapses. SYN1 in the pathogenesis of non-syndromic intellectual disability, showing that alterations of synaptic vesicle trafficking are one possible cause of this disease, and suggest that distinct mutations in SYN1 may lead to distinct brain pathologies.

Product Categories/Family for anti-SYN1 antibody

G Protein-coupled Receptors Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 19924099
• NCBI GeneID: 6853
• NCBI Accession #: NP_008881.2
• NCBI GenBank Nucleotide #: NM_006950.3
• UniProt Accession #: P17600; O75825; Q5H9A9; B1AJQ1
• Molecular Weight: 74,111 Da
• NCBI Official Full Name: synapsin-1 isoform Ia
• NCBI Official Synonym Full Names: synapsin I
• NCBI Official Symbol: SYN1
• NCBI Official Synonym Symbols: SYNI; SYN1a; SYN1b
• NCBI Protein Information: synapsin-1; brain protein 4.1
• UniProt Protein Name: Synapsin-1
• Protein Family: Synapsin
• UniProt Gene Name: SYN1
• UniProt Entry Name: SYN1_HUMAN

NCBI Description

This gene is a member of the synapsin gene family. Synapsins encode neuronal phosphoproteins which associate with the cytoplasmic surface of synaptic vesicles. Family members are characterized by common protein domains, and they are implicated in synaptogenesis and the modulation of neurotransmitter release, suggesting a potential role in several neuropsychiatric diseases. This member of the synapsin family plays a role in regulation of axonogenesis and synaptogenesis. The protein encoded serves as a substrate for several different protein kinases and phosphorylation may function in the regulation of this protein in the nerve terminal. Mutations in this gene may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

SYN1: neuronal phosphoprotein which associates with the cytoplasmic surface of synaptic vesicles and binds to the cytoskeleton. May function in the regulation of neurotransmitter release and of axonogenesis and synaptogenesis. Mutations may be associated with X-linked disorders with primary neuronal degeneration such as Rett syndrome. Two differentially spiced isoforms have been reported.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: Xp11.23

Cellular Component: Golgi apparatus; synaptic vesicle; dendrite; cytosol; cell junction

Molecular Function: protein binding; transporter activity; actin binding; protein kinase binding; calcium-dependent protein binding; catalytic activity; ATP binding

Biological Process: synaptic transmission; metabolic process; neurotransmitter secretion; regulation of neurotransmitter secretion

Disease: Epilepsy, X-linked, With Variable Learning Disabilities And Behavior Disorders