Nogo Receptor/RTN4R Cell Lysate | RTN4R cell lysate
Mouse Nogo Receptor/RTN4R HEK293 Overexpression Lysate
Gene Names
Rtn4r; NgR; NgR1; NOGOR
Applications
Western Blot
Synonyms
Nogo Receptor/RTN4R; Mouse Nogo Receptor/RTN4R HEK293 Overexpression Lysate; Mouse Nogo Receptor/NOGOR/RTN4R HEK293 Cell Lysate (WB positive control); reticulon 4 receptor; Mouse NgR Overexpression Lysate; Mouse NgR1 Overexpression Lysate; Mouse NOGOR Overexpression Lysate; Mouse Rtn4r Overexpression Lysate; RTN4R cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
669aa
Sequence
Met1-Ser447
Applicable Applications for RTN4R cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the the mature form of mouse RTN4R (NP_075358.1) (Met 1-Ser 447) was fused with the polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for RTN4R cell lysate
Reticulon 4 receptor (RTN4R), also known as Nogo-66 Receptor (NgR), is a glycosylphosphoinositol (GPI)-anchored protein that belongs to the Nogo recptor family including three members. Mouse RTN4R cDNA contains 1 LRP (Leucine-rich) repeats. RTN4R is expressed predominantly in neurons and their axons in the central nervous systems (CNS). As a receptor for myelin-derived proteins Nogo, myelin-associated glycoprotein (MAG), and myelin oligodendrocyte glycoprotein (OMG), RTN4R mediates axonal growth inhibition and may play a role in regulating axonal regeneration and plasticity in the adult CNS. It has been shown that RTN4R performs its inhibitory actions by interacting with the p75 neurotrophin receptor (p75NTR), a TNFRSF member also known for modulating the activities of the Trk family and for inducing apoptosis in neurons and oligodendrocytes. RTN4R may be proposed as a potential drug target for treatment of various neurological conditions such as spinal cord injury, CNS lesions, peripheral nerve injury, stroke and Alzheimer's disease (AD). Additionally, RTN4R may play a role in regulating the function of the gap junctions.
This Mouse Nogo Receptor/RTN4R overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Nogo Receptor/RTN4R protein from the overexpression lysate was verified.
This Mouse Nogo Receptor/RTN4R overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of Nogo Receptor/RTN4R protein from the overexpression lysate was verified.
References
1. Wang, X. et al., 2006, Ann Neurol. 60(5): 540-549. 2. Wang, Y.Z. et al., 2006, Neuroreport.17(6):605-609. 3. Zhu, H.Y. et al., 2007, Hum Pathol. 38(3): 426-434. 4. David, S. et al., 2008, Trends Neurosci. 31(5): 221-226. 5. Jiang, W. et al., 2009, Transl Res. 154(1): 40-48. 6. Zhang, L. et al., 2009, J Neurosci, 9(19): 6348-6352.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
50,987 Da
NCBI Official Full Name
reticulon-4 receptor
NCBI Official Synonym Full Names
reticulon 4 receptor
NCBI Official Symbol
Rtn4r
NCBI Official Synonym Symbols
NgR; NgR1; NOGOR
NCBI Protein Information
reticulon-4 receptor; nogo receptor; Nogo-66 receptor
UniProt Protein Name
Reticulon-4 receptor
Protein Family
UniProt Gene Name
Rtn4r
UniProt Synonym Gene Names
Nogor; NgR
UniProt Entry Name
RTN4R_MOUSE
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Product Notes
The RTN4R rtn4r (Catalog #AAA8116382) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 669aa. AAA Biotech's Nogo Receptor/RTN4R can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the RTN4R rtn4r for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Ser44 7. It is sometimes possible for the material contained within the vial of "Nogo Receptor/RTN4R, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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