MEGF10 cell lysate
Human MEGF10 HEK293 Overexpression Lysate
Gene Names
MEGF10; EMARDD
Applications
Western Blot
Synonyms
MEGF10; Human MEGF10 HEK293 Overexpression Lysate; Human MEGF10 HEK293 Cell Lysate (WB positive control); multiple EGF-like-domains 10; Human EMARDD Overexpression Lysate; MEGF10 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
843aa
Sequence
Met1-Gly857
Applicable Applications for MEGF10 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human MEGF10 (NP_115822.1) (Met1-Gly857) was expressed with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for MEGF10 cell lysate
MEGF10 (Multiple EGF Like Domains 10) is a Protein Coding gene. 2 alternatively spliced human isoforms have been reported. This gene encodes a member of the multiple epidermal growth factor-like domains protein family. The encoded protein plays a role in cell adhesion, motility, and proliferation, and is a critical mediator of apoptotic cell phagocytosis as well as amyloid-beta peptide uptake in the brain. MEGF10 may also function in the mosaic spacing of specific neuron subtypes in the retina through homotypic retinal neuron repulsion. It belongs to the MEGF family. Diseases associated with MEGF10 include Myopathy, Areflexia, Respiratory Distress, And Dysphagia, Early-Onset, and Dysphagia.
This Human MEGF10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of MEGF10 protein from the overexpression lysate was verified.
This Human MEGF10 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of MEGF10 protein from the overexpression lysate was verified.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
60,797 Da
NCBI Official Full Name
multiple epidermal growth factor-like domains protein 10 isoform a
NCBI Official Synonym Full Names
multiple EGF like domains 10
NCBI Official Symbol
MEGF10
NCBI Official Synonym Symbols
EMARDD
NCBI Protein Information
multiple epidermal growth factor-like domains protein 10
UniProt Protein Name
Multiple epidermal growth factor-like domains protein 10
Protein Family
UniProt Gene Name
MEGF10
UniProt Synonym Gene Names
KIAA1780; Multiple EGF-like domains protein 10
UniProt Entry Name
MEG10_HUMAN
NCBI Description
This gene encodes a member of the multiple epidermal growth factor-like domains protein family. The encoded protein plays a role in cell adhesion, motility and proliferation, and is a critical mediator of apoptotic cell phagocytosis as well as amyloid-beta peptide uptake in the brain. Expression of this gene may be associated with schizophrenia, and mutations in this gene are a cause of early-onset myopathy, areflexia, respiratory distress, and dysphagia (EMARDD) as well as congenital myopathy with minicores. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Apr 2012]
Research Articles on MEGF10
Similar Products
Product Notes
The MEGF10 megf10 (Catalog #AAA8115632) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 843aa. AAA Biotech's MEGF10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the MEGF10 megf10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly85 7. It is sometimes possible for the material contained within the vial of "MEGF10, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
