LYVE1 cell lysate
Mouse LYVE1 HEK293 Overexpression Lysate
Gene Names
Lyve1; Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik
Applications
Western Blot
Synonyms
LYVE1; Mouse LYVE1 HEK293 Overexpression Lysate; Mouse LYVE1/LYVE-1 HEK293 Cell Lysate (WB positive control); lymphatic vessel endothelial hyaluronan receptor 1; Mouse 1200012G08Rik Overexpression Lysate; Mouse Crsbp-1 Overexpression Lysate; Mouse Lyve-1 Overexpression Lysate; Mouse Xlkd1 Overexpression Lysate; LYVE1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
446aa
Sequence
Met1-Gly228
Applicable Applications for LYVE1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse LYVE1 (Q8BHC0) (Met1-Gly228) was expressed, fused with the Fc region of human IgG1 at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for LYVE1 cell lysate
LYVE1, also known as LYVE-1, is a type I integral membrane glycoprotein. It contains 1 Link domain and is mainly expressed in endothelial cells lining lymphatic vessels. LYVE1 acts as a receptor and binds to both soluble and immobilized hyaluronan. It may function in lymphatic hyaluronan transport and have a role in tumor metastasis. LYVE1 also is a cell surface receptor on lymphatic endothelial cells that can be used as a lymphatic endothelial cell marker, and sort these cells for experimental purposes. It also functions as a ligand-specific transporter trafficking between intracellular organelles and the plasma membrane. It plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding. It may act as a hyaluronan transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
This Mouse LYVE1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LYVE1 protein from the overexpression lysate was verified.
This Mouse LYVE1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LYVE1 protein from the overexpression lysate was verified.
References
Jackson DG. (2003) The lymphatics revisited: new perspectives from the hyaluronan receptor LYVE-1. Trends Cardiovasc Med. 13(1):1-7.Banerji S, et al. (1999) LYVE-1, a new homologue of the CD44 glycoprotein, is a lymph-specific receptor for hyaluronan. J Cell Biol. 144(4):789-801.Mouta Carreira C, et al. (2001) LYVE-1 is not restricted to the lymph vessels: expression in normal liver blood sinusoids and down-regulation in human liver cancer and cirrhosis. Cancer Res. 61(22): 8079-84.Cursiefen C, et al. (2002) Lymphatic vessels in vascularized human corneas: immunohistochemical investigation using LYVE-1 and podoplanin. Invest Ophthalmol Vis Sci. 43(7):2127-35.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,574 Da
NCBI Official Full Name
lymphatic vessel endothelial hyaluronic acid receptor 1
NCBI Official Synonym Full Names
lymphatic vessel endothelial hyaluronan receptor 1
NCBI Official Symbol
Lyve1
NCBI Official Synonym Symbols
Xlkd1; Lyve-1; Crsbp-1; 1200012G08Rik
NCBI Protein Information
lymphatic vessel endothelial hyaluronic acid receptor 1; cell surface retention sequence binding protein-1; cell surface retention sequence-binding protein 1; extra cellular link domain-containing 1; extracellular link domain-containing protein 1; lymphat
UniProt Protein Name
Lymphatic vessel endothelial hyaluronic acid receptor 1
Protein Family
UniProt Gene Name
Lyve1
UniProt Synonym Gene Names
Crsbp1; Xlkd1; LYVE-1; CRSBP-1
UniProt Entry Name
LYVE1_MOUSE
Uniprot Description
XLKD1: Ligand-specific transporter trafficking between intracellular organelles (TGN) and the plasma membrane. Plays a role in autocrine regulation of cell growth mediated by growth regulators containing cell surface retention sequence binding (CRS). May act as an hyaluronan (HA) transporter, either mediating its uptake for catabolism within lymphatic endothelial cells themselves, or its transport into the lumen of afferent lymphatic vessels for subsequent re-uptake and degradation in lymph nodes.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis
Cellular Component: membrane; integral to membrane; plasma membrane
Molecular Function: transmembrane receptor activity; hyaluronic acid binding
Biological Process: glycosaminoglycan catabolic process; transport; cell adhesion; signal transduction
Research Articles on LYVE1
Similar Products
Product Notes
The LYVE1 lyve1 (Catalog #AAA8116345) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 446aa. AAA Biotech's LYVE1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the LYVE1 lyve1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Gly22 8. It is sometimes possible for the material contained within the vial of "LYVE1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
