LCN1 cell lysate
Human LCN1 HEK293 Overexpression Lysate
Gene Names
LCN1; TP; TLC; PMFA; VEGP
Applications
Western Blot
Synonyms
LCN1; Human LCN1 HEK293 Overexpression Lysate; Human LCN1/VEGP/Lipocalin-1 HEK293 Cell Lysate (WB positive control); lipocalin 1; Human Lipocalin 1 Overexpression Lysate; Human MGC71975 Overexpression Lysate; Human PMFA Overexpression Lysate; Human TLC Overexpression Lysate; Human TP Overexpression Lysate; Human VEGP Overexpression Lysate; LCN1 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
169aa
Sequence
Met1-Asp176
Applicable Applications for LCN1 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the human LCN1 (NP_002288.1) extracellular domain (Met 1-Asp176) was fused with a polyhistidine tag at the C-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for LCN1 cell lysate
Lipocalin-1, also known as Von Ebner gland protein, VEG protein, Tear Prealbumin, VEGP, Tear lipocalin, and LCN1 is a secreted protein that belongs to the calycin superfamily and Lipocalin family. Human Lipocalin-1/VEGP was originally described as a major protein of human tear fluid, which was thought to be tear specific. Lipocalin-1/VEGP is identical to lingual von Ebner's gland protein and is also produced in the prostate, nasal mucosa, and tracheal mucosa. Homologous proteins have been found in the rat, pig, and probably dog and horse. Lipocalin-1/VEGP is an unusual lipocalin member, because of its high promiscuity for relative insoluble lipids and binding characteristics that differ from other members. Lipocalin-1/VEGP acts as the principal lipid-binding protein in tear fluid, a more general physiological function has to be proposed due to its wide distribution and properties. Lipocalin-1/VEGP would be ideally suited for scavenging of lipophilic, potentially harmful substances and thus might act as a general protection factor of epithelia. Lipocalin-1/LCN1 could play a role in taste reception. It could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Lipocalin-1/LCN1 can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. It exhibits an extremely wide ligand pocket.
This Human LCN1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LCN1 protein from the overexpression lysate was verified.
This Human LCN1 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of LCN1 protein from the overexpression lysate was verified.
References
Lassagne H. et al., 1993, Exp. Eye Res. 56:605-609. Redl, B. et al., 2000, Biochim Biophys Acta 1482 (1-2):241-8. Wojnar P. et al., 2001, J. Biol. Chem. 276:20206-20212. Wojnar P. et al., 2003, J. Biol. Chem. 278:16209-16215. Breustedt D.A. et al., 2005, J. Biol. Chem. 280:484-493.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
19,250 Da
NCBI Official Full Name
lipocalin-1 isoform 1
NCBI Official Synonym Full Names
lipocalin 1
NCBI Official Symbol
LCN1
NCBI Official Synonym Symbols
TP; TLC; PMFA; VEGP
NCBI Protein Information
lipocalin-1; VEG protein; tear prealbumin; Von Ebner gland protein; lipocalin 1 (tear prealbumin); protein migrating faster than albumin
UniProt Protein Name
Lipocalin-1
Protein Family
UniProt Gene Name
LCN1
UniProt Synonym Gene Names
VEGP; Tlc; TP; VEG protein
UniProt Entry Name
LCN1_HUMAN
NCBI Description
This gene encodes a member of the lipocalin family of small secretory proteins. Lipocalins are extracellular transport proteins that bind to a variety of hydrophobic ligands. The encoded protein is the primary lipid binding protein in tears and is overproduced in response to multiple stimuli including infection and stress. The encoded protein may be a marker for chromosome aneuploidy as well as an autoantigen in Sjogren's syndrome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and two pseudogenes of this gene are also located on the long arm of chromosome 9. [provided by RefSeq, Nov 2011]
Uniprot Description
LCN1: Could play a role in taste reception. Could be necessary for the concentration and delivery of sapid molecules in the gustatory system. Can bind various ligands, with chemical structures ranging from lipids and retinoids to the macrocyclic antibiotic rifampicin and even to microbial siderophores. Exhibits an extremely wide ligand pocket. Belongs to the calycin superfamily. Lipocalin family.
Protein type: Secreted; Inhibitor; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 9q34
Cellular Component: extracellular space; extracellular region
Molecular Function: cysteine protease inhibitor activity
Biological Process: long-chain fatty acid transport; retinal homeostasis; sensory perception of taste; response to stimulus; proteolysis; transmembrane transport
Research Articles on LCN1
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Product Notes
The LCN1 lcn1 (Catalog #AAA8114593) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 169aa. AAA Biotech's LCN1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the LCN1 lcn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Met1-Asp17 6. It is sometimes possible for the material contained within the vial of "LCN1, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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