IFNAR2 active protein

IFNAR2 Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120229

• Gene Names: IFNAR2; IFN-R; IMD45; IFNABR; IFNARB; IFN-alpha-REC
• Purity: >90% as determined by SDS-PAGE
• Synonyms: IFNAR2; IFNAR2 Protein; Human; Recombinant (hFc Tag); Human IFNAR2/IFNABR Protein (Fc Tag); IFN-alpha-REC Protein; IFN-R Protein; IFNABR Protein; IFNARB Protein; interferon (alpha; beta and omega) receptor 2; IFNAR2 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Lys243

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Ile 27
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the extracellular domain of human IFNAR2 isoform a (NP_997468.1)(Met 1-Lys 243) was fused with the Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Activity: 1. Measured by its ability to inhibit rh IFNbeta mediated protection of WISH Human amnion cells infected with vesicular stomatitis virus (VSV) to viral lysis. The EC50 for this effect is typically 0.2-1.2ug/mL.; 2. Measured by its binding ability in a functional ELISA. Immobilized PPICZa-IFNA2 at 10ug/mL (100uL/well) can bind IFNaR2-Fc. The EC50 of IFNaR2-Fc is 60-200ng/mL.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its binding ability in a functional ELISA. Immobilized PPICZa-IFNA2 at 10 ug/mL (100 uL/well) can bind IFNaR2-Fc, the EC50 of IFNaR2-Fc is 60-200 ng/mL.)

Related Product Information for IFNAR2 active protein

Background: Interferon-alpha/beta receptor beta chain (IFNAR2) is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulate Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Initial cell-surface IFNAR2 expression at test assessed by flow cytometry was widely distributed but showed overall significantly higher expression in CML patients when compared with normal controls. In 15 fresh patients who subsequently received IFNalpha therapy, IFNAR2 expression at test was significantly higher in cytogenetic good responders than in poor responders. Down-regulation of IFNAR2 expression during IFNalpha therapy was observed only in good responders but not in poor responders. The encoded protein also functions as an antiviral factor. IFNAR2 may associate with IFNAR1 to form the type I interferon receptor. This protein serves as a receptor for interferons alpha and beta. IFNAR2 is also involved in IFN-mediated STAT1, STAT2, and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Following binding of IFNalpha2, IFNAR2 is internalized, but, instead of being routed towards degradation as it is when complexed to IFNbeta, it recycles back to the cell surface.

References

Ito K, et al. (2004) Initial expression of interferon alpha receptor 2 (IFNAR2) on CD34-positive cells and its down-regulation correlate with clinical response to interferon therapy in chronic myelogenous leukemia. Eur J Haematol. 73(3): 191-205.Kim SH, et al. (1997) Mammalian type I interferon receptors consists of two subunits: IFNaR1 and IFNaR2. Gene. 196(1-2): 279-86.Saleh AZ, et al. (2004) Regulated proteolysis of the IFNaR2 subunit of the interferon-alpha receptor. Oncogene. 23(42): 7076-86.

NCBI and Uniprot Product Information

• NCBI GI #: 46488937
• NCBI GeneID: 3455
• NCBI Accession #: NP_997468.1
• NCBI GenBank Nucleotide #: NM_207585.2
• UniProt Accession #: P48551; Q15467; Q6FHD7; A8KAJ4; D3DSE8; D3DSE9
• Molecular Weight: 27,384 Da
• NCBI Official Full Name: interferon alpha/beta receptor 2 isoform a
• NCBI Official Synonym Full Names: interferon alpha and beta receptor subunit 2
• NCBI Official Symbol: IFNAR2
• NCBI Official Synonym Symbols: IFN-R; IMD45; IFNABR; IFNARB; IFN-alpha-REC
• NCBI Protein Information: interferon alpha/beta receptor 2
• UniProt Protein Name: Interferon alpha/beta receptor 2
• Protein Family: Interferon alpha/beta receptor
• UniProt Gene Name: IFNAR2
• UniProt Synonym Gene Names: IFNABR; IFNARB; IFN-R-2; IFN-alpha binding protein; IFN-alpha/beta receptor 2
• UniProt Entry Name: INAR2_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. Multiple transcript variants encoding at least two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IFNAR2: Associates with IFNAR1 to form the type I interferon receptor. Receptor for interferons alpha and beta. Involved in IFN-mediated STAT1, STAT2 and STAT3 activation. Isoform 1 and isoform 2 are directly involved in signal transduction due to their association with the TYR kinase, JAK1. Isoform 3 is a potent inhibitor of type I IFN receptor activity. Belongs to the type II cytokine receptor family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, cytokine; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q22.11

Cellular Component: extracellular space; integral to plasma membrane; plasma membrane; extracellular region

Molecular Function: protein binding; interferon-alpha/beta receptor activity; interferon-alpha/beta binding; protein kinase binding

Biological Process: regulation of transcription from RNA polymerase II promoter; cell proliferation; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; response to virus; JAK-STAT cascade

Disease: Hepatitis B Virus, Susceptibility To