COQ7 cell lysate
Human COQ7 HEK293 Overexpression Lysate
Gene Names
COQ7; CAT5; CLK1; CLK-1
Applications
Western Blot
Synonyms
COQ7; Human COQ7 HEK293 Overexpression Lysate; Human COQ7 HEK293 Cell Lysate (WB positive control); coenzyme Q7 homolog; ubiquinone (yeast); Human CAT5 Overexpression Lysate; Human CLK-1 Overexpression Lysate; Human CLK1 Overexpression Lysate; COQ7 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
192aa
Sequence
Ser37-Leu217
Applicable Applications for COQ7 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Human
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mature form of human COQ7 isoform 1 (Q99807-1) (Ser 37-Leu 217) was fused with a polyhistidine tag at the C-terminus and a signal peptide at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for COQ7 cell lysate
Ubiquinone biosynthesis protein COQ7 homolog, also known as Coenzyme Q biosynthesis protein 7 homolog, Timing protein clk-1 homolog and COQ7, is a mitochondrion inner membrane and peripheral membrane protein which belongs to theCOQ7 family. It is expressed dominantly in heart and skeletal muscle. COQ7 is synthesized as a preprotein that is imported into the mitochondrial matrix, where the sequence is cleaved off and the mature protein becomes loosely associated with the inner membrane. This enzyme is responsible for the hydroxylation of 5-demethoxyubiquinone to 5-hydroxyubiquinone. Human COQ7 protein is mostly helical, and contains an alpha-helical membrane insertion. It has a potential N-glycosylation site, a phosphorylation site for protein kinase C and another for casein kinase II, and three N-myristoylation sites. COQ7 is involved in lifespan determination in ubiquinone-independent manner. It is also involved in ubiquinone biosynthesis. COQ7 is potential central metabolic regulator.
This Human COQ7 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of COQ7 protein from the overexpression lysate was verified.
This Human COQ7 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of COQ7 protein from the overexpression lysate was verified.
References
Ewbank J.J., et al., 1997, Science. 275: 980-3. Vajo Z., et al., 1999, Mamm. Genome. 10: 1000-4. Wiemann S., et al., 2001, Genome Res. 11: 422-35. Stenmark, P. et al., 2001, J Biol Chem. 276 (36): 33297-300. Martin J., et al., 2004, Nature. 432: 988-94.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
217
NCBI Official Full Name
Ubiquinone biosynthesis protein COQ7 homolog
NCBI Official Synonym Full Names
coenzyme Q7 homolog, ubiquinone (yeast)
NCBI Official Symbol
COQ7
NCBI Official Synonym Symbols
CAT5; CLK1; CLK-1
NCBI Protein Information
ubiquinone biosynthesis protein COQ7 homolog; placental protein KG-20; timing protein clk-1 homolog; COQ7 coenzyme Q, 7 homolog ubiquinone; coenzyme Q biosynthesis protein 7 homolog
UniProt Protein Name
Ubiquinone biosynthesis protein COQ7 homolog
Protein Family
UniProt Gene Name
COQ7
UniProt Entry Name
COQ7_HUMAN
NCBI Description
The protein encoded by this gene is similar to a mitochondrial di-iron containing hydroxylase in Saccharomyces cerevisiae that is involved with ubiquinone biosynthesis. Mutations in the yeast gene lead to slower development and longer life span. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
COQ7: Involved in lifespan determination in ubiquinone- independent manner. Involved in ubiquinone biosynthesis. Potential central metabolic regulator. Belongs to the COQ7 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Cofactor and Vitamin Metabolism - ubiquinone and other terpenoid-quinone biosynthesis; Mitochondrial
Chromosomal Location of Human Ortholog: 16p12.3
Cellular Component: mitochondrial inner membrane; nucleus
Molecular Function: metal ion binding
Biological Process: ubiquinone biosynthetic process; neurogenesis; in utero embryonic development; determination of adult life span; neural tube formation; age-dependent response to oxidative stress; organelle ATP synthesis coupled electron transport
Research Articles on COQ7
Similar Products
Product Notes
The COQ7 coq7 (Catalog #AAA8114921) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 192aa. AAA Biotech's COQ7 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the COQ7 coq7 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ser37-Leu2 17. It is sometimes possible for the material contained within the vial of "COQ7, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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