APRIL/TNFSF13 Cell Lysate | TNFSF13 cell lysate
Mouse APRIL/TNFSF13 HEK293 Overexpression Lysate
Gene Names
Tnfsf13; April; Tall2; Trdl1; 2310026N09Rik
Applications
Western Blot
Synonyms
APRIL/TNFSF13; Mouse APRIL/TNFSF13 HEK293 Overexpression Lysate; Mouse TNFSF13 HEK293 Cell Lysate (WB positive control); tumor necrosis factor (ligand) superfamily; member 13; Mouse 2310026N09Rik Overexpression Lysate; Mouse April Overexpression Lysate; Mouse Tall2 Overexpression Lysate; Mouse Trdl1 Overexpression Lysate; TNFSF13 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
405aa
Sequence
Ala96-Leu240
Applicable Applications for TNFSF13 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the mouse TNFSF13 (Q9D777) (Ala96-Leu240) was expressed with the fused Fc region of human IgG1 at the N-terminus.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TNFSF13 cell lysate
TNFSF13 is a member of the tumor necrosis factor (TNF) ligand family. It is a ligand for TNFRSF17/BCMA. TNFSF13 is lowly expressed in normal tissues, but is elevated in several types of tumors and transformed cell lines. It is important for B cell development. TNFSF13 may also play a role in T-independent type II antigen responses and T cell survival, and induce proliferation/survival of non lymphoid cells. It exists as a functional homotrimer. It can bind to two cell surface receptors, BCMA and TACI, which it shares with BAFF to exert downstream T-and B-cell regulatory effects. TNFSF13 also has been demonstrated to bind to proteoglycans on the cell surface.
This Mouse APRIL/TNFSF13 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of APRIL/TNFSF13 protein from the overexpression lysate was verified.
This Mouse APRIL/TNFSF13 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of APRIL/TNFSF13 protein from the overexpression lysate was verified.
References
Bossen C, et al. (2007) BAFF, APRIL and their receptors: structure, function and signaling. Semin. Immunol. 18(5):263-75.Tangye SG, et al. (2007) BAFF, APRIL and human B cell disorders. Immunol. 18(5): 305-17.Osman W, et al. (2012) Association of common variants in TNFRSF13B, TNFSF13, and ANXA3 with serum levels of non-albumin protein and immunoglobulin isotypes in Japanese. PLoS One. 7(4):e32683.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
26,889 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 13 isoform 2
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 13
NCBI Official Symbol
Tnfsf13
NCBI Official Synonym Symbols
April; Tall2; Trdl1; 2310026N09Rik
NCBI Protein Information
tumor necrosis factor ligand superfamily member 13; a proliferation-inducing ligand
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 13
Protein Family
UniProt Gene Name
Tnfsf13
UniProt Synonym Gene Names
April; APRIL
UniProt Entry Name
TNF13_MOUSE
Uniprot Description
TNFSF13: Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. May be implicated in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes. Belongs to the tumor necrosis factor family. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine
Cellular Component: extracellular space; membrane; cytoplasm; extracellular region; external side of plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor binding
Biological Process: regulation of immune response; immune system process; immunoglobulin production in mucosal tissue; positive regulation of cell proliferation; positive regulation of germinal center formation; immune response; immunoglobulin mediated immune response; positive regulation of isotype switching to IgA isotypes
Research Articles on TNFSF13
Similar Products
Product Notes
The TNFSF13 tnfsf13 (Catalog #AAA8116961) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 405aa. AAA Biotech's APRIL/TNFSF13 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TNFSF13 tnfsf13 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Ala96-Leu2 40. It is sometimes possible for the material contained within the vial of "APRIL/TNFSF13, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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