4-1BBL/TNFSF9 Cell Lysate | TNFSF9 cell lysate
Mouse 4-1BBL/TNFSF9 HEK293 Overexpression Lysate
Gene Names
Tnfsf9; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
Applications
Western Blot
Synonyms
4-1BBL/TNFSF9; Mouse 4-1BBL/TNFSF9 HEK293 Overexpression Lysate; Mouse 4-1BBL/CD137L/TNFSF9 HEK293 Cell Lysate (WB positive control); tumor necrosis factor (ligand) superfamily; member 9; Mouse 4-1BB-L Overexpression Lysate; Mouse 4-1BBL Overexpression Lysate; Mouse AI848817 Overexpression Lysate; Mouse Cd137l Overexpression Lysate; Mouse Ly63l Overexpression Lysate; TNFSF9 cell lysate
Host
HEK293 Cells
Form/Format
1x Sample Buffer (1x modified RIPA buffer+1x SDS loading buffer).
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Sequence Positions
222aa
Sequence
Arg104-Glu309
Applicable Applications for TNFSF9 cell lysate
Western Blot (WB)
Application Notes
WB: Use at an assay dependent dilution.
Species
Mouse
Recommend Usage
1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200uL pure water and boil for 2-5 min.
Sequence Construction
A DNA sequence encoding the extracellular domain (Arg 104-Glu 309) of mouse TNFSF9 (NP_033430.1) precursor was expressed with a N-terminal polyhistidine tag.
Preparation Method
Cell lysate was prepared by homogenization of the over-expressed cells in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay (protein assay, Microplate Standard assay). The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Preparation and Storage
Store at 4 degree C for up to twelve months from date of receipt. After re-dissolution, aliquot and store at -80 degree C for up to twelve months. Avoid repeated freeze-thaw cycles.
Samples are stable for up to twelve months from date of receipt.
Samples are stable for up to twelve months from date of receipt.
Related Product Information for TNFSF9 cell lysate
4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
This Mouse 4-1BBL/TNFSF9 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of 4-1BBL/TNFSF9 protein from the overexpression lysate was verified.
This Mouse 4-1BBL/TNFSF9 overexpression lysate was created in HEK293 Cells and intented for use as a Western blot (WB) positive control. Purification of 4-1BBL/TNFSF9 protein from the overexpression lysate was verified.
Product Categories/Family for TNFSF9 cell lysate
References
Cheung CT, (2007) Neutralizing anti-4-1BBL treatment improves cardiac function in viral myocarditis. Lab Invest. 87(7): 651-61.Wang C, et al. (2009) Immune regulation by 4-1BB and 4-1BBL: complexities and challenges. Immunol Rev. 229(1): 192-215.Habib-Agahi M, et al. (2009) 4-1BBL costimulation retrieves CD28 expression in activated T cells. Cell Immunol. 256(1-2): 39-46.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,853 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 9
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 9
NCBI Official Symbol
Tnfsf9
NCBI Official Synonym Symbols
Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
NCBI Protein Information
tumor necrosis factor ligand superfamily member 9; 4-1BB ligand
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 9
Protein Family
UniProt Gene Name
Tnfsf9
UniProt Synonym Gene Names
Cd137l; Cd157l; Ly63l; 4-1BBL
UniProt Entry Name
TNFL9_MOUSE
Uniprot Description
TNFL9: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. Belongs to the tumor necrosis factor family.
Protein type: Apoptosis; Cytokine; Membrane protein, integral; Cell cycle regulation
Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding
Biological Process: positive regulation of interferon-gamma production; positive regulation of cytotoxic T cell differentiation; cell-cell signaling; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; myeloid dendritic cell differentiation; immune response; positive regulation of activated T cell proliferation; regulation of cell proliferation
Research Articles on TNFSF9
Similar Products
Product Notes
The TNFSF9 tnfsf9 (Catalog #AAA8116347) is a Cell Lysate produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 222aa. AAA Biotech's 4-1BBL/TNFSF9 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: Use at an assay dependent dilution. Researchers should empirically determine the suitability of the TNFSF9 tnfsf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Arg104-Glu 309. It is sometimes possible for the material contained within the vial of "4-1BBL/TNFSF9, Cell Lysate" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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