Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse 4-1BBL(TNFSF9) expression on RAW264.7 cells. Cells were stained with APC-conjugated anti-Mouse 4-1BBL(TNFSF9). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Rabbit anti-Mouse 4-1BBL/TNFSF9 Monoclonal Antibody | anti-TNFSF9 antibody
Recombinant Anti-4-1BBL/TNFSF9 Antibody (APC), Rabbit Monoclonal
Gene Names
Tnfsf9; Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Purity
Protein A
Synonyms
4-1BBL/TNFSF9; Monoclonal Antibody; Recombinant Anti-4-1BBL/TNFSF9 Antibody (APC); Rabbit Monoclonal; 4-1BBL/CD137L/TNFSF9 Antibody (APC); Rabbit MAb; tumor necrosis factor (ligand) superfamily; member 9; Anti-4-1BB-L Antibody; Anti-4-1BBL Antibody; Anti-AI848817 Antibody; Anti-Cd137l Antibody; Anti-Ly63l Antibody; anti-TNFSF9 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
014
Specificity
Mouse 4-1BBL/CD137L/TNFSF9
Purity/Purification
Protein A
Form/Format
Liquid; Aqueous solution containing 0.5% BSA and 0.09% sodium azide
Concentration
10ul/Test, 0.1mg/ml (varies by lot)
Applicable Applications for anti-TNFSF9 antibody
Flow Cytometry (FC/FACS/FCM)
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse 4-1BBL/CD137L/TNFSF9 protein
Conjugation
APC
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse 4-1BBL/CD137L/TNFSF9 (rM 4-1BBL/CD137L/TNFSF9) and conjugated with APC under optimum conditions, the unreacted APC was removed.
Preparation and Storage
This antibody can be stored at 2-8 degree C for twelve months without detectable loss of activity. Protected from prolonged exposure to light. Do not freeze! Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal.
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse 4-1BBL(TNFSF9) expression on RAW264.7 cells. Cells were stained with APC-conjugated anti-Mouse 4-1BBL(TNFSF9). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of Mouse 4-1BBL(TNFSF9) expression on RAW264.7 cells. Cells were stained with APC-conjugated anti-Mouse 4-1BBL(TNFSF9). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)
Related Product Information for anti-TNFSF9 antibody
4-1BB ligand is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. It is shown to be a type II surface glycoprotein belonging to the TNF superfamily. Expression of 4-1BBL is restricted to APCs, such as dendritic cells, macrophages, and activated B cells. Members of the TNF-TNF receptor superfamily have been shown to play critical roles in regulating cellular activation, differentiation and apoptosis. Several studies have reported that 4-1BBL/4-1BB interaction provided a co-stimulatory signal to T cells, and increased T cell proliferation and cytokines production. In addition, 4-1BBL is involved in cancers, infectious diseases and autoimmune diseases.
Product Categories/Family for anti-TNFSF9 antibody
References
Cheung CT, (2007) Neutralizing anti-4-1BBL treatment improves cardiac function in viral myocarditis. Lab Invest. 87(7): 651-61.Wang C, et al. (2009) Immune regulation by 4-1BB and 4-1BBL: complexities and challenges. Immunol Rev. 229(1): 192-215.Habib-Agahi M, et al. (2009) 4-1BBL costimulation retrieves CD28 expression in activated T cells. Cell Immunol. 256(1-2): 39-46.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
33,853 Da
NCBI Official Full Name
tumor necrosis factor ligand superfamily member 9
NCBI Official Synonym Full Names
tumor necrosis factor (ligand) superfamily, member 9
NCBI Official Symbol
Tnfsf9
NCBI Official Synonym Symbols
Ly63l; 4-1BBL; Cd137l; 4-1BB-L; AI848817
NCBI Protein Information
tumor necrosis factor ligand superfamily member 9; 4-1BB ligand
UniProt Protein Name
Tumor necrosis factor ligand superfamily member 9
Protein Family
UniProt Gene Name
Tnfsf9
UniProt Synonym Gene Names
Cd137l; Cd157l; Ly63l; 4-1BBL
UniProt Entry Name
TNFL9_MOUSE
Uniprot Description
TNFL9: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. Belongs to the tumor necrosis factor family.
Protein type: Apoptosis; Cytokine; Membrane protein, integral; Cell cycle regulation
Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane
Molecular Function: cytokine activity; tumor necrosis factor receptor superfamily binding; tumor necrosis factor receptor binding; receptor binding
Biological Process: positive regulation of interferon-gamma production; positive regulation of cytotoxic T cell differentiation; cell-cell signaling; positive regulation of interleukin-12 production; positive regulation of interleukin-6 production; myeloid dendritic cell differentiation; immune response; positive regulation of activated T cell proliferation; regulation of cell proliferation
Research Articles on TNFSF9
Similar Products
Product Notes
The TNFSF9 tnfsf9 (Catalog #AAA8110894) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-4-1BBL/TNFSF9 Antibody (APC), Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's 4-1BBL/TNFSF9 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS/FCM). Researchers should empirically determine the suitability of the TNFSF9 tnfsf9 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "4-1BBL/TNFSF9, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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