ApoA-1 Active Protein | ApoA1 active protein

Human CellExp ApoA-1, human recombinant

Cat. Num. AAA844794

• Applications: SDS-Page
• Purity: >=95%
• Synonyms: ApoA-1; Human CellExp ApoA-1; human recombinant; Apolipoprotein A-I; APOA1; MGC117399; ApoA1 active protein

• Host: HEK293 cells
• Purity/Purification: >=95%
• Form/Format: Lyophilized from 0.22 mum filtered solution in PBS, pH7.4. Generally 5-8% Mannitol or trehalose is added as a protectant before lyophilization.; Appearance: Lyophilized
• Sequence Length: 267

• Applicable Applications for ApoA1 active protein: SDS-PAGE
• Solubility/Reconstitution Instructions: Centrifuge the vial prior to opening. Reconstitute in sterile deionized water to a concentration of 50 ug/ml. Solubilize for 30 to 60 min. at RT with occasional gentle mixing. Do not vortex. Carrier protein (0.1% HAS or BSA) is strongly recommended for further dilution and long term storage.
• Endotoxin Level: <1 EU/mug by LAL method
• Biological Activity: Immobilized Human ApoAI at 10 mug/mL (100 mul/well) can bind biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 10-100 ng/mL.
• Results: Immobilized Human ApoAI at 10 mug/mL (100 mul/well) can bind biotinylated human SCARB1. The EC50 of biotinylated human SCARB1 is 10-100 ng/mL.
• Handling: Centrifuge the vial prior to opening.
• Preparation and Storage: At -20 degree C; Shelf Life: 12 months

Testing Data

Related Product Information for ApoA1 active protein

Background: ApoA1 is also known as apolipoprotein A-I, ApoA-I, and is the major protein component of high density lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism. Chylomicrons secreted from the intestinal enterocyte also contain ApoA1 but it is quickly transferred to HDL in the bloodstream. The protein promotes cholesterol efflux from tissues to the liver for excretion. It is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. ApoA-I was also isolated as a prostacyclin (PGI2) stabilizing factor, and thus may have an anticlotting effect. Defects in the gene encoding it are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. In addition, it has been shown that ApoA1 is implicated in the anti-endotoxin function of HDL via interaction with lipopolysaccharide or endotoxin.

Product Categories/Family for ApoA1 active protein

Proteins and Enzymes; Human cell expressed recombinant proteinsCytokines; Growth Factors & Hormones; Human cell expressed recombinant proteinsProteins and Enzymes; ApolipoproteinsProteins and Enzymes; Proteins and Enzymes (A-Z)

NCBI and Uniprot Product Information

• NCBI GI #: 4557321
• NCBI GeneID: 335
• NCBI Accession #: NP_000030.1
• NCBI GenBank Nucleotide #: NM_000039.1
• UniProt Accession #: P02647; Q6LDN9; Q6Q785; Q9UCS8; Q9UCT8; A8K866
• Molecular Weight: This protein is fused with polyhistidine tag at the C-terminus, and has a calculated MW of 29 kDa. The predicted N-terminus is Asp 25. DTT-reduced Protein migrates as 26-30 kDa in SDS-PAGE.
• NCBI Official Full Name: apolipoprotein A-I preproprotein
• NCBI Official Synonym Full Names: apolipoprotein A-I
• NCBI Official Symbol: APOA1
• NCBI Protein Information: apolipoprotein A-I
• UniProt Protein Name: Apolipoprotein A-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOA1
• UniProt Synonym Gene Names: Apo-AI; ApoA-I; ProapoA-I
• UniProt Entry Name: APOA1_HUMAN

NCBI Description

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq, Jul 2008]

Uniprot Description

APOA1: Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. Interacts with APOA1BP and CLU. Component of a sperm activating protein complex (SPAP), consisting of APOA1, an immunoglobulin heavy chain, an immunoglobulin light chain and albumin. Interacts with NDRG1. Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. The oxidized form at Met-110 and Met-136 is increased in individuals with increased risk for coronary artery disease, such as in carrier of the eNOSa/b genotype and exposure to cigarette smoking. It is also present in increased levels in aortic lesions relative to native ApoA-I and increased levels are seen with increasing severity of disease. Belongs to the apolipoprotein A1/A4/E family.

Protein type: Vesicle; Lipid-binding; Endoplasmic reticulum; Secreted, signal peptide; Secreted; Cell development/differentiation; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 11q23-q24

Cellular Component: extracellular space; chylomicron; cell surface; endoplasmic reticulum lumen; endocytic vesicle; early endosome; extracellular region; plasma membrane; cytoplasmic vesicle; nucleus; cytosol; vesicle

Molecular Function: identical protein binding; protein binding; enzyme binding; phospholipid transporter activity; lipase inhibitor activity; chemorepellent activity; cholesterol transporter activity; beta-amyloid binding; cholesterol binding; phospholipid binding; phosphatidylcholine binding; high-density lipoprotein binding; apolipoprotein A-I receptor binding; apolipoprotein receptor binding

Biological Process: phototransduction, visible light; negative chemotaxis; negative regulation of lipase activity; axon regeneration in the peripheral nervous system; negative regulation of interleukin-1 beta secretion; sequestering of lipid; regulation of cholesterol absorption; transforming growth factor beta receptor signaling pathway; positive regulation of stress fiber formation; response to drug; cholesterol metabolic process; platelet activation; organ regeneration; regulation of Cdc42 protein signal transduction; adrenal gland development; positive regulation of hydrolase activity; positive regulation of Rho protein signal transduction; lipoprotein metabolic process; positive regulation of transferase activity; vitamin transport; cholesterol biosynthetic process; negative regulation of cytokine secretion during immune response; cholesterol homeostasis; response to estrogen stimulus; lipoprotein biosynthetic process; peptidyl-methionine modification; phosphatidylcholine biosynthetic process; positive regulation of lipoprotein lipase activity; blood vessel endothelial cell migration; cellular lipid metabolic process; platelet degranulation; phospholipid efflux; retinoid metabolic process; transmembrane transport; response to nutrient; phospholipid homeostasis; integrin-mediated signaling pathway; positive regulation of fatty acid biosynthetic process; receptor-mediated endocytosis; regulation of protein amino acid phosphorylation; cholesterol transport; protein amino acid oxidation; protein stabilization; negative regulation of heterotypic cell-cell adhesion; neurite regeneration; cholesterol efflux; G-protein coupled receptor protein signaling pathway; glucocorticoid metabolic process; reverse cholesterol transport; endothelial cell proliferation; negative regulation of inflammatory response; blood coagulation

Disease: Hypoalphalipoproteinemia, Primary; Amyloidosis, Familial Visceral