Western Blot (WB)
(MBS423052(0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat c-FOS Antibody | anti-FOS antibody
Goat anti-c-FOS (aa283-295) Antibody
Western Blot: Approx 37kDa band observed in lysates of cell line HeLa (calculated MW of 40.7kDa according to NP_005243.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour.
IHC: Paraffin embedded Human Tonsil. Recommended concentration: 8ug/ml
Immunofluorescence : Strong expression of the protein seen in the nucleusof HeLa cells, and in the ER and cytoplasm of MCF7 cells. Recommended concentration : 10ug/mL.
Western Blot (WB)
(MBS423052(0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
(MBS423052(0.3ug/ml) staining of HeLa lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Immunohistochemistry (IHC)
(MBS423052 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunohistochemistry (IHC)
(MBS423052 (5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.)
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml))
Immunofluorescence (IF)
(Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing strong nuclear and weak cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml))
Immunofluorescence (IF)
( Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunofluorescence (IF)
( Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing endoplasmic reticulum and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).)
Immunohistochemistry (IHC)
((8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
((8µg/ml) staining of paraffin embedded Human Tonsil. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.)
Immunohistochemistry (IHC)
( Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
Immunohistochemistry (IHC)
( Negative Control showing staining of paraffin embedded Human Tonsil, with no primary antibody.)
NCBI and Uniprot Product Information
NCBI Description
The Fos gene family consists of 4 members: FOS, FOSB, FOSL1, and FOSL2. These genes encode leucine zipper proteins that can dimerize with proteins of the JUN family, thereby forming the transcription factor complex AP-1. As such, the FOS proteins have been implicated as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of the FOS gene has also been associated with apoptotic cell death. [provided by RefSeq, Jul 2008]
Uniprot Description
Fos: a proto-oncogenic transcription factor of the bZIP family. Dimerizes with proteins of the JUN family, thereby forming the transcription factor complex AP-1. FOS proteins function as regulators of cell proliferation, differentiation, and transformation. In some cases, expression of FOS has also been associated with apoptotic cell death. Expression increases upon a variety of stimuli, including growth factors, cytokines, neurotransmitters, polypeptide hormones, stress and cell injury.
Protein type: DNA-binding; Motility/polarity/chemotaxis; Oncoprotein; Transcription factor
Chromosomal Location of Human Ortholog: 14q24.3
Cellular Component: nucleoplasm; nucleus
Molecular Function: protein binding; transcription factor activity; transcription factor binding
Biological Process: DNA methylation; inflammatory response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter; transforming growth factor beta receptor signaling pathway
Research Articles on FOS
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Product Notes
The FOS fos (Catalog #AAA423052) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-c-FOS (aa283-295) Antibody reacts with Tested: Human; Expected from sequence similarity: Human, Dog, Pig, Cow and may cross-react with other species as described in the data sheet. AAA Biotech's c-FOS can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB), Immunofluroescence (IF), Immunohistochemistry (IHC). Peptide ELISA: Antibody detection limit dilution 1: 128000. Western Blot: Approx 37kDa band observed in lysates of cell line HeLa (calculated MW of 40.7kDa according to NP_005243.1). Recommended concentration: 0.3-1ug/ml. Primary incubation was 1 hour. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 8ug/ml Immunofluorescence : Strong expression of the protein seen in the nucleusof HeLa cells, and in the ER and cytoplasm of MCF7 cells. Recommended concentration : 10ug/mL. Researchers should empirically determine the suitability of the FOS fos for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: SETARSVPDM DLS. It is sometimes possible for the material contained within the vial of "c-FOS, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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